Loading…

Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung

Gastrin-releasing peptide (GRP), the mammalian homologue of the amphibian peptide bombesin, is present in pulmonary neuroendocrine cells and appears to be a growth factor for both normal and neoplastic pulmonary cells. Previously we have reported the cloning of the messenger RNAs (mRNAs) and gene th...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of clinical investigation 1987-10, Vol.80 (4), p.1172-1179
Main Authors: SPINDEL, E. R, SUNDAY, M. E, HOFLER, H, WOLFE, H. J, HABENER, J. F, CHIN, W. W
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c398t-3530e21ee84d9767d6bd3b75458e9babad765e4ebc2c281247aad5308101064f3
cites
container_end_page 1179
container_issue 4
container_start_page 1172
container_title The Journal of clinical investigation
container_volume 80
creator SPINDEL, E. R
SUNDAY, M. E
HOFLER, H
WOLFE, H. J
HABENER, J. F
CHIN, W. W
description Gastrin-releasing peptide (GRP), the mammalian homologue of the amphibian peptide bombesin, is present in pulmonary neuroendocrine cells and appears to be a growth factor for both normal and neoplastic pulmonary cells. Previously we have reported the cloning of the messenger RNAs (mRNAs) and gene that encode human GRP. We now report that GRP mRNAs are markedly elevated in human fetal lung during the canalicular phase of pulmonary development (from approximately 16 to 30 wk gestation). By RNA blot and in situ hybridization analyses, GRP mRNAs were first detectable in fetal lung at 9-10 wk, plateaued at levels 25-fold higher than in adult lungs from 16 to approximately 30 wk and then declined to near adult levels by 34 wk gestation. By contrast, GRP peptide levels remain elevated until several months after birth. Consistent with this, in situ hybridization and immunohistochemical studies showed that GRP mRNA and peptide consistently colocalized in early gestation lung but that in neonatal lung, many cells that contained GRP peptide no longer contained GRP mRNA. The transient expression of high levels of GRP mRNAs during an approximately 12-wk phase of fetal lung development suggests that the secretion of GRP or its COOH-terminal peptides from pulmonary neuroendocrine cells may play a role in normal lung development.
doi_str_mv 10.1172/JCI113176
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_442362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81013962</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-3530e21ee84d9767d6bd3b75458e9babad765e4ebc2c281247aad5308101064f3</originalsourceid><addsrcrecordid>eNpVkUtv1DAUhS1EVYbCgh-A5AVCQiIlfiROFiyqEY-iCiRU1taNc5MxSuxgO4NY9a_joaNRWfnK5zv3oUPIC1ZeMqb4uy_ba8YEU_UjsmFV1RQNF81jsilLzopWieYJeRrjz7JkUlbynJyLupKtUhtydxvARYsuUZxwD8l6R_1AZ4wR3YiBfv96RdEZ31s30hFiCtYVIcMQDz8LLsn2-JYCXdaU_XvMxTR7B-EPHYP_nXZ0AJN8oNbR3TqDowMmmOi0uvEZORtgivj8-F6QHx8_3G4_FzffPl1vr24KI9omFaISJXKG2Mi-VbXq664Xnapk1WDbQQe9qiuU2BlueMO4VAB99jSsZGUtB3FB3t_3XdZuxt7kgwNMegl2zntqD1b_rzi706Pfaym5qHn2vz76g_-1Ykx6ttHgNIFDv0Z9GCTaf-Cbe9AEH2PA4TSDlfoQlj6FldmXD5c6kcd0sv7qqEM0MA05KmPjCVOqVVK04i-iKJ8r</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81013962</pqid></control><display><type>article</type><title>Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung</title><source>PubMed Central (Training)</source><source>EZB Electronic Journals Library</source><creator>SPINDEL, E. R ; SUNDAY, M. E ; HOFLER, H ; WOLFE, H. J ; HABENER, J. F ; CHIN, W. W</creator><creatorcontrib>SPINDEL, E. R ; SUNDAY, M. E ; HOFLER, H ; WOLFE, H. J ; HABENER, J. F ; CHIN, W. W</creatorcontrib><description>Gastrin-releasing peptide (GRP), the mammalian homologue of the amphibian peptide bombesin, is present in pulmonary neuroendocrine cells and appears to be a growth factor for both normal and neoplastic pulmonary cells. Previously we have reported the cloning of the messenger RNAs (mRNAs) and gene that encode human GRP. We now report that GRP mRNAs are markedly elevated in human fetal lung during the canalicular phase of pulmonary development (from approximately 16 to 30 wk gestation). By RNA blot and in situ hybridization analyses, GRP mRNAs were first detectable in fetal lung at 9-10 wk, plateaued at levels 25-fold higher than in adult lungs from 16 to approximately 30 wk and then declined to near adult levels by 34 wk gestation. By contrast, GRP peptide levels remain elevated until several months after birth. Consistent with this, in situ hybridization and immunohistochemical studies showed that GRP mRNA and peptide consistently colocalized in early gestation lung but that in neonatal lung, many cells that contained GRP peptide no longer contained GRP mRNA. The transient expression of high levels of GRP mRNAs during an approximately 12-wk phase of fetal lung development suggests that the secretion of GRP or its COOH-terminal peptides from pulmonary neuroendocrine cells may play a role in normal lung development.</description><identifier>ISSN: 0021-9738</identifier><identifier>EISSN: 1558-8238</identifier><identifier>DOI: 10.1172/JCI113176</identifier><identifier>PMID: 3654977</identifier><identifier>CODEN: JCINAO</identifier><language>eng</language><publisher>Ann Arbor, MI: American Society for Clinical Investigation</publisher><subject>Biological and medical sciences ; Cloning, Molecular ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development ; Fundamental and applied biological sciences. Psychology ; Gastrin-Releasing Peptide ; Gestational Age ; Humans ; Immunohistochemistry ; Lung - embryology ; Lung - metabolism ; Nucleic Acid Hybridization ; Organogenesis. Fetal development ; Organogenesis. Physiological fonctions ; Peptides - genetics ; RNA, Messenger - metabolism</subject><ispartof>The Journal of clinical investigation, 1987-10, Vol.80 (4), p.1172-1179</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-3530e21ee84d9767d6bd3b75458e9babad765e4ebc2c281247aad5308101064f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC442362/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC442362/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=7797439$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3654977$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SPINDEL, E. R</creatorcontrib><creatorcontrib>SUNDAY, M. E</creatorcontrib><creatorcontrib>HOFLER, H</creatorcontrib><creatorcontrib>WOLFE, H. J</creatorcontrib><creatorcontrib>HABENER, J. F</creatorcontrib><creatorcontrib>CHIN, W. W</creatorcontrib><title>Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>Gastrin-releasing peptide (GRP), the mammalian homologue of the amphibian peptide bombesin, is present in pulmonary neuroendocrine cells and appears to be a growth factor for both normal and neoplastic pulmonary cells. Previously we have reported the cloning of the messenger RNAs (mRNAs) and gene that encode human GRP. We now report that GRP mRNAs are markedly elevated in human fetal lung during the canalicular phase of pulmonary development (from approximately 16 to 30 wk gestation). By RNA blot and in situ hybridization analyses, GRP mRNAs were first detectable in fetal lung at 9-10 wk, plateaued at levels 25-fold higher than in adult lungs from 16 to approximately 30 wk and then declined to near adult levels by 34 wk gestation. By contrast, GRP peptide levels remain elevated until several months after birth. Consistent with this, in situ hybridization and immunohistochemical studies showed that GRP mRNA and peptide consistently colocalized in early gestation lung but that in neonatal lung, many cells that contained GRP peptide no longer contained GRP mRNA. The transient expression of high levels of GRP mRNAs during an approximately 12-wk phase of fetal lung development suggests that the secretion of GRP or its COOH-terminal peptides from pulmonary neuroendocrine cells may play a role in normal lung development.</description><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic and Fetal Development</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrin-Releasing Peptide</subject><subject>Gestational Age</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Lung - embryology</subject><subject>Lung - metabolism</subject><subject>Nucleic Acid Hybridization</subject><subject>Organogenesis. Fetal development</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Peptides - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0021-9738</issn><issn>1558-8238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpVkUtv1DAUhS1EVYbCgh-A5AVCQiIlfiROFiyqEY-iCiRU1taNc5MxSuxgO4NY9a_joaNRWfnK5zv3oUPIC1ZeMqb4uy_ba8YEU_UjsmFV1RQNF81jsilLzopWieYJeRrjz7JkUlbynJyLupKtUhtydxvARYsuUZxwD8l6R_1AZ4wR3YiBfv96RdEZ31s30hFiCtYVIcMQDz8LLsn2-JYCXdaU_XvMxTR7B-EPHYP_nXZ0AJN8oNbR3TqDowMmmOi0uvEZORtgivj8-F6QHx8_3G4_FzffPl1vr24KI9omFaISJXKG2Mi-VbXq664Xnapk1WDbQQe9qiuU2BlueMO4VAB99jSsZGUtB3FB3t_3XdZuxt7kgwNMegl2zntqD1b_rzi706Pfaym5qHn2vz76g_-1Ykx6ttHgNIFDv0Z9GCTaf-Cbe9AEH2PA4TSDlfoQlj6FldmXD5c6kcd0sv7qqEM0MA05KmPjCVOqVVK04i-iKJ8r</recordid><startdate>19871001</startdate><enddate>19871001</enddate><creator>SPINDEL, E. R</creator><creator>SUNDAY, M. E</creator><creator>HOFLER, H</creator><creator>WOLFE, H. J</creator><creator>HABENER, J. F</creator><creator>CHIN, W. W</creator><general>American Society for Clinical Investigation</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19871001</creationdate><title>Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung</title><author>SPINDEL, E. R ; SUNDAY, M. E ; HOFLER, H ; WOLFE, H. J ; HABENER, J. F ; CHIN, W. W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-3530e21ee84d9767d6bd3b75458e9babad765e4ebc2c281247aad5308101064f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrin-Releasing Peptide</topic><topic>Gestational Age</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Lung - embryology</topic><topic>Lung - metabolism</topic><topic>Nucleic Acid Hybridization</topic><topic>Organogenesis. Fetal development</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Peptides - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SPINDEL, E. R</creatorcontrib><creatorcontrib>SUNDAY, M. E</creatorcontrib><creatorcontrib>HOFLER, H</creatorcontrib><creatorcontrib>WOLFE, H. J</creatorcontrib><creatorcontrib>HABENER, J. F</creatorcontrib><creatorcontrib>CHIN, W. W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SPINDEL, E. R</au><au>SUNDAY, M. E</au><au>HOFLER, H</au><au>WOLFE, H. J</au><au>HABENER, J. F</au><au>CHIN, W. W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung</atitle><jtitle>The Journal of clinical investigation</jtitle><addtitle>J Clin Invest</addtitle><date>1987-10-01</date><risdate>1987</risdate><volume>80</volume><issue>4</issue><spage>1172</spage><epage>1179</epage><pages>1172-1179</pages><issn>0021-9738</issn><eissn>1558-8238</eissn><coden>JCINAO</coden><abstract>Gastrin-releasing peptide (GRP), the mammalian homologue of the amphibian peptide bombesin, is present in pulmonary neuroendocrine cells and appears to be a growth factor for both normal and neoplastic pulmonary cells. Previously we have reported the cloning of the messenger RNAs (mRNAs) and gene that encode human GRP. We now report that GRP mRNAs are markedly elevated in human fetal lung during the canalicular phase of pulmonary development (from approximately 16 to 30 wk gestation). By RNA blot and in situ hybridization analyses, GRP mRNAs were first detectable in fetal lung at 9-10 wk, plateaued at levels 25-fold higher than in adult lungs from 16 to approximately 30 wk and then declined to near adult levels by 34 wk gestation. By contrast, GRP peptide levels remain elevated until several months after birth. Consistent with this, in situ hybridization and immunohistochemical studies showed that GRP mRNA and peptide consistently colocalized in early gestation lung but that in neonatal lung, many cells that contained GRP peptide no longer contained GRP mRNA. The transient expression of high levels of GRP mRNAs during an approximately 12-wk phase of fetal lung development suggests that the secretion of GRP or its COOH-terminal peptides from pulmonary neuroendocrine cells may play a role in normal lung development.</abstract><cop>Ann Arbor, MI</cop><pub>American Society for Clinical Investigation</pub><pmid>3654977</pmid><doi>10.1172/JCI113176</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0021-9738
ispartof The Journal of clinical investigation, 1987-10, Vol.80 (4), p.1172-1179
issn 0021-9738
1558-8238
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_442362
source PubMed Central (Training); EZB Electronic Journals Library
subjects Biological and medical sciences
Cloning, Molecular
Embryology: invertebrates and vertebrates. Teratology
Embryonic and Fetal Development
Fundamental and applied biological sciences. Psychology
Gastrin-Releasing Peptide
Gestational Age
Humans
Immunohistochemistry
Lung - embryology
Lung - metabolism
Nucleic Acid Hybridization
Organogenesis. Fetal development
Organogenesis. Physiological fonctions
Peptides - genetics
RNA, Messenger - metabolism
title Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A12%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transient%20elevation%20of%20messenger%20RNA%20encoding%20gastrin-releasing%20peptide,%20a%20putative%20pulmonary%20growth%20factor%20in%20human%20fetal%20lung&rft.jtitle=The%20Journal%20of%20clinical%20investigation&rft.au=SPINDEL,%20E.%20R&rft.date=1987-10-01&rft.volume=80&rft.issue=4&rft.spage=1172&rft.epage=1179&rft.pages=1172-1179&rft.issn=0021-9738&rft.eissn=1558-8238&rft.coden=JCINAO&rft_id=info:doi/10.1172/JCI113176&rft_dat=%3Cproquest_pubme%3E81013962%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c398t-3530e21ee84d9767d6bd3b75458e9babad765e4ebc2c281247aad5308101064f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=81013962&rft_id=info:pmid/3654977&rfr_iscdi=true