Production, purification and crystallization of a trans-sialidase from Trypanosoma vivax

Sialidases and trans‐sialidases play important roles in the life cycles of various microorganisms. These enzymes can serve nutritional purposes, act as virulence factors or mediate cellular interactions (cell evasion and invasion). In the case of the protozoan parasite Trypanosoma vivax, trans‐siali...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology communications Structural biology communications, 2015-05, Vol.71 (5), p.577-585
Main Authors: Haynes, Carole L. F., Ameloot, Paul, Remaut, Han, Callewaert, Nico, Sterckx, Yann G.-J., Magez, Stefan
Format: Article
Language:English
Subjects:
Crystallization
Crystallography, X-Ray
Glycoproteins - chemistry
Glycoproteins - isolation & purification
Molecular Parasitology
Neuraminidase - chemistry
Neuraminidase - isolation & purification
trans-sialidase
Trypanosoma vivax
Trypanosoma vivax - chemistry
Trypanosoma vivax - enzymology
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Summary:Sialidases and trans‐sialidases play important roles in the life cycles of various microorganisms. These enzymes can serve nutritional purposes, act as virulence factors or mediate cellular interactions (cell evasion and invasion). In the case of the protozoan parasite Trypanosoma vivax, trans‐sialidase activity has been suggested to be involved in infection‐associated anaemia, which is the major pathology in the disease nagana. The physiological role of trypanosomal trans‐sialidases in host–parasite interaction as well as their structures remain obscure. Here, the production, purification and crystallization of a recombinant version of T. vivaxtrans‐sialidase 1 (rTvTS1) are described. The obtained rTvTS1 crystals diffracted to a resolution of 2.5 Å and belonged to the orthorhombic space group P212121, with unit‐cell parameters a = 57.3, b = 78.4, c = 209.0 Å.
ISSN:2053-230X
2053-230X
DOI:10.1107/S2053230X15002496