An Unprecedented NADPH Domain Conformation in Lysine Monooxygenase NbtG Provides Insights into Uncoupling of Oxygen Consumption from Substrate Hydroxylation

• Published in: The Journal of biological chemistry 2015-05, Vol.290 (20), p.12676-12688
• Main Authors: Binda, Claudia, Robinson, Reeder M., Martin del Campo, Julia S., Keul, Nicholas D., Rodriguez, Pedro J., Robinson, Howard H., Mattevi, Andrea, Sobrado, Pablo
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; C4a-hydroperoxyflavin; Crystallography, X-Ray; Enzymology; flavin; Flavin-Adenine Dinucleotide - chemistry; Flavin-Adenine Dinucleotide - genetics; Flavin-Adenine Dinucleotide - metabolism; flavin-dependent monooxygenase; flavoprotein; Hydroxylation; iron metabolism; Lysine - chemistry; Lysine - genetics; Lysine - metabolism; lysine monooxygenase; Mixed Function Oxygenases - chemistry; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - metabolism; N-hydroxylating monooxygenases; NADP - chemistry; NADP - genetics; NADP - metabolism; Nocardia - enzymology; Nocardia - genetics; Oxygen Consumption - physiology; Protein Structure, Tertiary; siderophore; virulence factor
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M114.629485

N-Hydroxylating monooxygenases are involved in the biosynthesis of iron-chelating hydroxamate-containing siderophores that play a role in microbial virulence. These flavoenzymes catalyze the NADPH- and oxygen-dependent hydroxylation of amines such as those found on the side chains of lysine and ornithine. In this work we report the biochemical and structural characterization of Nocardia farcinica Lys monooxygenase (NbtG), which has similar biochemical properties to mycobacterial homologs. NbtG is also active on d-Lys, although it binds l-Lys with a higher affinity. Differently from the ornithine monooxygenases PvdA, SidA, and KtzI, NbtG can use both NADH and NADPH and is highly uncoupled, producing more superoxide and hydrogen peroxide than hydroxylated Lys. The crystal structure of NbtG solved at 2.4 Å resolution revealed an unexpected protein conformation with a 30° rotation of the NAD(P)H domain with respect to the flavin adenine dinucleotide (FAD) domain that precludes binding of the nicotinamide cofactor. This “occluded” structure may explain the biochemical properties of NbtG, specifically with regard to the substantial uncoupling and limited stabilization of the C4a-hydroperoxyflavin intermediate. Biological implications of these findings are discussed. Flavin-dependent lysine monooxygenases are involved in siderophore biosynthesis and are promising bacterial drug targets. Biochemical and structural characterization of lysine monooxygenase from Nocardia farcinica (NbtG) is presented. An unprecedented domain conformation blocks the proper binding of NAD(P)H in the active site, which explains the high level of uncoupling observed in NbtG. The structural and biochemical data should aid in drug design.