Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis

• Published in: BioMed research international 2015-01, Vol.2015 (2015), p.1-9
• Main Authors: Gonzalez-Rey, Elena, Delgado, Mario, Hernández-Cortés, Pedro, Aneiros-Fernández, Jose, Mesa, Francisco, Padial-Molina, Miguel, Roman, Maria, Galindo-Moreno, Pablo, Crespo-Lora, Vicente, Peregrina, Magdalena, O'Valle, Francisco, Aguilar, David
• Format: Article
• Language: English
• Subjects: Animals; Arthritis; Arthritis, Experimental - genetics; Arthritis, Experimental - physiopathology; Arthritis, Rheumatoid - genetics; Arthritis, Rheumatoid - pathology; Bone surgery; Care and treatment; Cell cycle; Cell Differentiation - genetics; Cell division; Cell growth; Collagen; Colleges & universities; Cytokines; Dentistry; Disease Models, Animal; Dosage and administration; Etanercept; Gangrene; Humans; Laboratories; Mice; Molecular weight; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; Physiological aspects; Proteins; RNA-Binding Proteins - biosynthesis; RNA-Binding Proteins - genetics; Rodents; Stem cells; Stem Cells - metabolism
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2015/681456

Background. Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein. Methods. CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1. Results. Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization ( P < 0.001 ). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions ( P < 0.0001 ) and was related to the severity of the lesions (Spearman’s rho = 0.775, P = 0.0001 ). Conclusions. Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair.