Altered versican cleavage in ADAMTS5 deficient mice; A novel etiology of myxomatous valve disease

• Published in: Developmental biology 2011-09, Vol.357 (1), p.152-164
• Main Authors: Dupuis, Loren E., McCulloch, Daniel R., McGarity, Jessica D., Bahan, Alexandria, Wessels, Andy, Weber, Deidra, Diminich, A. Megan, Nelson, Courtney M., Apte, Suneel S., Kern, Christine B.
• Format: Article
• Language: English
• Subjects: ADAM Proteins - genetics; ADAM Proteins - metabolism; ADAMTS5; ADAMTS5 Protein; adults; Animals; Bone Morphogenetic Protein 2 - genetics; Bone Morphogenetic Protein 2 - metabolism; Cell Proliferation; Endocardial cushions; Endocardium - metabolism; etiology; Extracellular matrix; Gene Expression Regulation, Developmental; heart; Heart - embryology; Heart Valve Diseases - etiology; Heart Valve Diseases - genetics; Heart Valves - embryology; Heart Valves - metabolism; heterozygosity; Mesoderm - metabolism; metalloproteinases; Mice; Mice, Transgenic; Myxomatous valves; penetrance; proteoglycans; Versican; Versicans - metabolism
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/j.ydbio.2011.06.041

In fetal valve maturation the mechanisms by which the relatively homogeneous proteoglycan-rich extracellular matrix (ECM) of endocardial cushions is replaced by a specialized and stratified ECM found in mature valves are not understood. Therefore, we reasoned that uncovering proteases critical for ‘remodeling’ the proteoglycan rich (extracellular matrix) ECM may elucidate novel mechanisms of valve development. We have determined that mice deficient in ADAMTS5, (A Disintegrin-like And Metalloprotease domain with ThromboSpondin-type 1 motifs) which we demonstrated is expressed predominantly by valvular endocardium during cardiac valve maturation, exhibited enlarged valves. ADAMTS5 deficient valves displayed a reduction in cleavage of its substrate versican, a critical cardiac proteoglycan. In vivo reduction of versican, in Adamts5−/− mice, achieved through Vcan heterozygosity, substantially rescued the valve anomalies. An increase in BMP2 immunolocalization, Sox9 expression and mesenchymal cell proliferation were observed in Adamts5−/− valve mesenchyme and correlated with expansion of the spongiosa (proteoglycan-rich) region in Adamts5−/− valve cusps. Furthermore, these data suggest that ECM remodeling via ADAMTS5 is required for endocardial to mesenchymal signaling in late fetal valve development. Although adult Adamts5−/− mice are viable they do not recover from developmental valve anomalies and have myxomatous cardiac valves with 100% penetrance. Since the accumulation of proteoglycans is a hallmark of myxomatous valve disease, based on these data we hypothesize that a lack of versican cleavage during fetal valve development may be a potential etiology of adult myxomatous valve disease. ► ADAMTS5 deficient mice develop enlarged cardiac valves by late fetal stages. ► Valve mesenchyme in Adamts5-/- mice display increased proliferation and intercellular space. ► In vivo reduction of the ADAMTS5 substrate versican rescues the valve phenotype in Adamts5-/- mice. ► Endothelial expression of ADAMTS5 is required for differentiation of cardiac valve mesenchyme. ► BMP2 and Sox9 expression is not down-regulated in Adamts5-/- cardiac valve cusps.