A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X

Terpenoid phytoalexins function as defense compound against a broad spectrum of pathogens and pests in the plant kingdom. However, the role of phytoalexin in antiviral defense is still elusive. In this study, we identified the biosynthesis pathway of a sesquiterpenoid phytoalexin, capsidiol 3-acetat...

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Bibliographic Details
Published in:Scientific reports 2015-05, Vol.5 (1), p.9682-9682, Article 9682
Main Authors: Li, Ran, Tee, Chuan-Sia, Jiang, Yu-Lin, Jiang, Xi-Yuan, Venkatesh, Prasanna Nori, Sarojam, Rajani, Ye, Jian
Format: Article
Language:English
Subjects:
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Acetic acid
Biosynthesis
Biosynthetic Pathways - genetics
Enzymatic activity
Gene Silencing
Humanities and Social Sciences
Jasmonic acid
multidisciplinary
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana - virology
Pathogens
Pests
Phytoalexins
Plant Diseases - virology
Plant Proteins - antagonists & inhibitors
Plant Proteins - genetics
Plant Proteins - metabolism
Plant viruses
Plasmids - genetics
Plasmids - metabolism
Potatoes
Potexvirus - drug effects
Potexvirus - metabolism
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
RNA viruses
Science
Sesquiterpenes - chemistry
Sesquiterpenes - metabolism
Sesquiterpenes - pharmacology
Signal Transduction - drug effects
Terpenes - chemistry
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Summary:Terpenoid phytoalexins function as defense compound against a broad spectrum of pathogens and pests in the plant kingdom. However, the role of phytoalexin in antiviral defense is still elusive. In this study, we identified the biosynthesis pathway of a sesquiterpenoid phytoalexin, capsidiol 3-acetate as an antiviral response against RNA virus Potato Virus X (PVX) in Nicotiana benthamiana . NbTPS1 and NbEAH genes were found strongly induced by PVX-infection. Enzymatic activity and genetic evidence indicated that both genes were involved in the PVX-induced biosynthesis of capsidiol 3-acetate. NbTPS1 - or NbEAH -silenced plant was more susceptible to PVX. The accumulation of capsidiol 3-acetate in PVX-infected plant was partially regulated by jasmonic acid signaling receptor COI1. These findings provide an insight into a novel mechanism of how plant uses the basal arsenal machinery to mount a fight against virus attack even in susceptible species.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep09682