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Xuebijing Injection Promotes M2 Polarization of Macrophages and Improves Survival Rate in Septic Mice
Xuebijing (XBJ) injection, a concoction of several Chinese herbs, has been widely used as animmunomodulator for the treatment of severe sepsis in China. However, the precise mechanismsresponsible for its efficacy have not been fully elucidated. In our study, we determined the flowcytometry markers (...
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Published in: | Evidence-based complementary and alternative medicine 2015-01, Vol.2015 (2015), p.1-9 |
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description | Xuebijing (XBJ) injection, a concoction of several Chinese herbs, has been widely used as animmunomodulator for the treatment of severe sepsis in China. However, the precise mechanismsresponsible for its efficacy have not been fully elucidated. In our study, we determined the flowcytometry markers (F4/80, CD11c, and CD206), the levels of secreted cytokines (TNF-α, IL-6, andIL-10), and the expression of specific proteins of M2 (Ym1, Fizz1, and Arg1) to assessmacrophage polarization. Treatment with XBJ lowered M1 associated cytokine levels andincreased the level of M2 associated cytokine level. The percentage of M2 phenotype cells of XBJgroup was much higher than that of the control group. Expressions of phosphorylated Janus kinase1 (JAK1) and signal transducer and activator of transcription 6 (STAT6) were markedly enhancedafter the administration of XBJ; on the other hand, the M2 associated cytokines and proteins weredecreased following treatment with JAK1 or STAT6 inhibitor. In addition, the treatment of XBJsignificantly improved the survival rate of septic mice. These studies demonstrate that XBJ canmarkedly promote M2 polarization and improve the survival rate of septic mice, therebycontributing to therapeutic effect in the treatment of septic complications. |
doi_str_mv | 10.1155/2015/352642 |
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However, the precise mechanismsresponsible for its efficacy have not been fully elucidated. In our study, we determined the flowcytometry markers (F4/80, CD11c, and CD206), the levels of secreted cytokines (TNF-α, IL-6, andIL-10), and the expression of specific proteins of M2 (Ym1, Fizz1, and Arg1) to assessmacrophage polarization. Treatment with XBJ lowered M1 associated cytokine levels andincreased the level of M2 associated cytokine level. The percentage of M2 phenotype cells of XBJgroup was much higher than that of the control group. Expressions of phosphorylated Janus kinase1 (JAK1) and signal transducer and activator of transcription 6 (STAT6) were markedly enhancedafter the administration of XBJ; on the other hand, the M2 associated cytokines and proteins weredecreased following treatment with JAK1 or STAT6 inhibitor. In addition, the treatment of XBJsignificantly improved the survival rate of septic mice. These studies demonstrate that XBJ canmarkedly promote M2 polarization and improve the survival rate of septic mice, therebycontributing to therapeutic effect in the treatment of septic complications.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2015/352642</identifier><identifier>PMID: 26064161</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Cytokines ; Genotype & phenotype ; Hospitals ; Infection ; Inflammation ; Inflammatory diseases ; Laboratory animals ; Macrophages ; Metabolic disorders ; Mortality ; Patient outcomes ; Sepsis ; Tumor necrosis factor ; Tumor necrosis factor-TNF</subject><ispartof>Evidence-based complementary and alternative medicine, 2015-01, Vol.2015 (2015), p.1-9</ispartof><rights>Copyright © 2015 Yan-Cun Liu et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Yan-Cun Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Yan-Cun Liu et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-48785a082bde30d5c232c9269f556d2b54ecb9b994be54c289f8f9d3bb726a553</citedby><cites>FETCH-LOGICAL-c528t-48785a082bde30d5c232c9269f556d2b54ecb9b994be54c289f8f9d3bb726a553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1709291223/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1709291223?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26064161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sali, Avni</contributor><creatorcontrib>Sheng, Zhi-Yong</creatorcontrib><creatorcontrib>Dong, Ning</creatorcontrib><creatorcontrib>Chai, Yan-Fen</creatorcontrib><creatorcontrib>Yao, Feng-Hua</creatorcontrib><creatorcontrib>Liu, Yan-Cun</creatorcontrib><creatorcontrib>Yao, Yong-Ming</creatorcontrib><title>Xuebijing Injection Promotes M2 Polarization of Macrophages and Improves Survival Rate in Septic Mice</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Xuebijing (XBJ) injection, a concoction of several Chinese herbs, has been widely used as animmunomodulator for the treatment of severe sepsis in China. 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These studies demonstrate that XBJ canmarkedly promote M2 polarization and improve the survival rate of septic mice, therebycontributing to therapeutic effect in the treatment of septic complications.</description><subject>Cytokines</subject><subject>Genotype & phenotype</subject><subject>Hospitals</subject><subject>Infection</subject><subject>Inflammation</subject><subject>Inflammatory diseases</subject><subject>Laboratory animals</subject><subject>Macrophages</subject><subject>Metabolic disorders</subject><subject>Mortality</subject><subject>Patient outcomes</subject><subject>Sepsis</subject><subject>Tumor necrosis factor</subject><subject>Tumor necrosis factor-TNF</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNks9rFDEYhgdRbK2evEvAiyhrk2-SSXIRSvHHQheLVegtZDLf7GaZTaaZmRX96826da1e9JSE9-Eh-fIWxVNGXzMmxClQJk5LARWHe8Uxk5zNOCh1_7CX10fFo2FYUwpaSvmwOIKKVpxV7LjA6wlrv_ZhSeZhjW70MZDLFDdxxIEsgFzGzib_3f4MYksW1qXYr-wyxzY0ZL7pU9zmw9WUtn5rO_LJjkh8IFfYj96RhXf4uHjQ2m7AJ7frSfHl3dvP5x9mFx_fz8_PLmZOgBpnXEklLFVQN1jSRjgowWmodCtE1UAtOLpa11rzGgV3oHSrWt2UdS2hskKUJ8Wbvbef6g02DsOYbGf65Dc2fTPRevNnEvzKLOPWcM6Z1ioLXtwKUryZcBjNxg8Ou84GjNNgmAQpKaWc_hutlMxKkCyjz_9C13FKIU8iC6kGzQDK39TSdmh8aGO-ottJzRkXquKClzvq1Z7K3zAMCdvD6xg1uz6YXR_Mvg-ZfnZ3IAf2VwEy8HIPrHxo7Ff_fzbMCLb2DpxVipc_AKQnxVQ</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Sheng, Zhi-Yong</creator><creator>Dong, Ning</creator><creator>Chai, Yan-Fen</creator><creator>Yao, Feng-Hua</creator><creator>Liu, Yan-Cun</creator><creator>Yao, Yong-Ming</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Xuebijing Injection Promotes M2 Polarization of Macrophages and Improves Survival Rate in Septic Mice</title><author>Sheng, Zhi-Yong ; 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However, the precise mechanismsresponsible for its efficacy have not been fully elucidated. In our study, we determined the flowcytometry markers (F4/80, CD11c, and CD206), the levels of secreted cytokines (TNF-α, IL-6, andIL-10), and the expression of specific proteins of M2 (Ym1, Fizz1, and Arg1) to assessmacrophage polarization. Treatment with XBJ lowered M1 associated cytokine levels andincreased the level of M2 associated cytokine level. The percentage of M2 phenotype cells of XBJgroup was much higher than that of the control group. Expressions of phosphorylated Janus kinase1 (JAK1) and signal transducer and activator of transcription 6 (STAT6) were markedly enhancedafter the administration of XBJ; on the other hand, the M2 associated cytokines and proteins weredecreased following treatment with JAK1 or STAT6 inhibitor. In addition, the treatment of XBJsignificantly improved the survival rate of septic mice. These studies demonstrate that XBJ canmarkedly promote M2 polarization and improve the survival rate of septic mice, therebycontributing to therapeutic effect in the treatment of septic complications.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26064161</pmid><doi>10.1155/2015/352642</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Cytokines Genotype & phenotype Hospitals Infection Inflammation Inflammatory diseases Laboratory animals Macrophages Metabolic disorders Mortality Patient outcomes Sepsis Tumor necrosis factor Tumor necrosis factor-TNF |
title | Xuebijing Injection Promotes M2 Polarization of Macrophages and Improves Survival Rate in Septic Mice |
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