Raman spectroscopy for grading of live osteosarcoma cells

Osteosarcoma is the most common primary malignant bone tumor, and the grading of osteosarcoma cells relies on traditional histopathology and molecular biology methods, which require RNA extraction, protein isolation and immunohistological staining. All these methods require cell isolation, lysis or...

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Bibliographic Details
Published in:Stem cell research & therapy 2015-04, Vol.6 (1), p.81-81, Article 81
Main Authors: Chiang, Yi-Hung, Wu, Stewart H, Kuo, Yi-Chun, Chen, How-Foo, Chiou, Arthur, Lee, Oscar K
Format: Article
Language:English
Subjects:
B cells
Basigin - metabolism
Bone Neoplasms - chemistry
Bone Neoplasms - metabolism
Bone Neoplasms - pathology
Cell Differentiation
Cell Line
Diagnosis
Durapatite - chemistry
Histochemistry
Humans
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 9 - metabolism
Mesenchymal Stromal Cells - chemistry
Mesenchymal Stromal Cells - metabolism
Methods
Molecular biology
Neoplasm Grading
Osteoblasts - chemistry
Osteoblasts - metabolism
Osteosarcoma
Osteosarcoma - chemistry
Osteosarcoma - metabolism
Osteosarcoma - pathology
Raman spectroscopy
Real-Time Polymerase Chain Reaction
RNA
RNA - analysis
RNA - metabolism
Spectrum Analysis, Raman
Stem cells
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Summary:Osteosarcoma is the most common primary malignant bone tumor, and the grading of osteosarcoma cells relies on traditional histopathology and molecular biology methods, which require RNA extraction, protein isolation and immunohistological staining. All these methods require cell isolation, lysis or fixation, which is time-consuming and requires certain amount of tumor specimen. In this study, we report the use of Raman spectroscopy for grading of malignant osteosarcoma cells. We demonstrate that, based on the detection of differential production of mineral species, Raman spectroscopy can be used as a live cell analyzer to accurately assess the grades of osteosarcoma cells by evaluating their mineralization levels. Mineralization level was assessed by measuring amount of hydroxyapatite (HA), which is highly expressed in mature osteoblasts, but not in poorly differentiated osteosarcoma cell or mesenchymal stem cells, the putative cell-of-origin of osteosarcoma. We found that under Raman spectroscopy, the level of HA production was high in MG-63 cells, which are low-grade. Moreover, hydroxyapatite production was low in high-grade osteosarcoma cells such as 143B and SaOS2 cells (p 
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-015-0074-5