Fermentative Production of Pyranone Derivate I from Marine Vibrio sp. SKMARSP9: Isolation, Characterization and Bioactivity Evaluation

Pyranone derivative I was isolated from fermented broth of isolated marine bacterial strain Vibrio sp. SKMARSP9. The compound I was characterized, and evaluated for its antimicrobial properties. The isolated strain was identified based on 16S rRNA based phylogenetic analysis. The molecular analysis...

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Bibliographic Details
Published in:Indian journal of microbiology 2015-09, Vol.55 (3), p.292-301
Main Authors: Shiva Krishna, P, Sudheer Kumar, B, Raju, P, Murty, M. S. R, Prabhakar Rao, T, Singara Charya, M. A, Prakasham, R. S
Format: Article
Language:English
Subjects:
anti-infective properties
antioxidant activity
Antioxidants
Bacteria
Biological activity
Biomedical and Life Sciences
Chloride
data analysis
electrospray ionization mass spectrometry
high performance liquid chromatography
hydrophobicity
Life Sciences
Liquid chromatography
Medical Microbiology
Metabolites
Microbiology
molecular models
Natural products
nuclear magnetic resonance spectroscopy
Original
Original Article
Pharmaceutical industry
phylogeny
Potassium
ribosomal RNA
Sodium
Sodium chloride
spectral analysis
Vibrio
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Summary:Pyranone derivative I was isolated from fermented broth of isolated marine bacterial strain Vibrio sp. SKMARSP9. The compound I was characterized, and evaluated for its antimicrobial properties. The isolated strain was identified based on 16S rRNA based phylogenetic analysis. The molecular analysis data suggested that this strain is closely related to Vibrio ruber, Vibrio sp. MSSRF10 and Vibrio rhizosphaerae. The best fermentative growth of this isolate was achieved under halophilic conditions and grew efficiently at 30 °C in the presence of 12 % NaCl. The compound I production by this strain is associated with growth. The unpurified extract is hydrophobic in nature, and released only during late growth phase. The extract was purified and characterized by spectral data using NMR, DEPT, and ESI–MS. The purity of I was 97 % which was confirmed by HPLC. The pyranone derivative I exhibited >50 % antioxidant activity and broad spectrum antimicrobial properties against gram negative and gram positive strains. Molecular docking analysis revealed that this pyranone derivative I may be a potential candidate at pharmaceutical sector.
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-015-0521-0