Evaluation of an Indirect-ELISA Test for Trypanosoma evansi Infection (Surra) in Buffaloes and Its Application to a Serological Survey in Thailand

Surra, caused by Trypanosoma evansi, is a neglected disease due to frequent subclinical evolution, especially in bovines in Asia. However, acute and chronic signs are regularly observed, with significant sanitary and economic impacts. In this study, we evaluated and applied an indirect-ELISA test fo...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-8
Main Authors: Jittapalapong, Sathaporn, Rodtian, Pranee, Leboucher, Emilye, Yangtara, Sarawut, Kamyingkird, Ketsarin, Desquesnes, Marc, Kocher, Arthur, Dargantes, Alan
Format: Article
Language:English
Subjects:
Animals
Antigens
Buffaloes - parasitology
Cattle
Diagnosis
Diseases
Elephantidae
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
Horses
Infections
Livestock
Methods
Mortality
Parasites
R&D
Research & development
Studies
Thailand
Trypanosoma - isolation & purification
Trypanosoma - pathogenicity
Trypanosoma evansi
Trypanosomiasis
Trypanosomiasis - diagnosis
Trypanosomiasis - pathology
Trypanosomiasis - veterinary
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Summary:Surra, caused by Trypanosoma evansi, is a neglected disease due to frequent subclinical evolution, especially in bovines in Asia. However, acute and chronic signs are regularly observed, with significant sanitary and economic impacts. In this study, we evaluated and applied an indirect-ELISA test for the detection of anti-T. evansi immunoglobulin G in buffaloes using antibovine conjugate. Based on buffalo reference sera from the Philippines, a two-graph receiver operating characteristics analysis (TG-ROC) was conducted to define an optimal cut-off value; sensitivity and specificity were estimated at 92.5% and 94.2%, respectively. A cross-sectional serological survey was carried out in the major buffalo breeding areas of Thailand; 892 buffaloes from 8 provinces were sampled in North, Northeastern, and Southern Thailand. Seropositive buffaloes were found in all 8 provinces, on 20.3% of farms for an overall prevalence of 12.2% (95% CI 10.2–14.5%). Nearly one-third of the sampled population was exposed to infection. Broader sampling would be necessary but is not possible in the southern half-wild breeding systems. According to our results, buffaloes may constitute a large and robust reservoir for T. evansi, which is a permanent threat to other livestock such as cattle and horses as well as wild animals such as elephants in Southest Asia.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/361037