Bacterial Community Composition in Oligosaline Lake Bosten: Low Overlap of Betaproteobacteria and Bacteroidetes with Freshwater Ecosystems

Oligosaline lakes in arid regions provide indispensable water resources for humans; however, information on the bacterial community composition (BCC) of this ecosystem is limited. In the present study, we explored seasonal and vertical variations in BCC in Lake Bosten, a unique oligosaline lake (1.2...

Full description

Saved in:
Bibliographic Details
Published in:Microbes and Environments 2015, Vol.30(2), pp.180-188
Main Authors: Tang, Xiangming, Xie, Guijuan, Shao, Keqiang, Dai, Jiangyu, Chen, Yuangao, Xu, Qiujin, Gao, Guang
Format: Article
Language:English
Subjects:
bacterial community composition
diversity
Lake Bosten
oligosaline
salinity
seasonal dynamics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c513t-4ada8e1b22ddb1a8073f915e7155b47a28fda77acc7fe0b1d9860f689e5c94503
cites cdi_FETCH-LOGICAL-c513t-4ada8e1b22ddb1a8073f915e7155b47a28fda77acc7fe0b1d9860f689e5c94503
container_end_page 188
container_issue 2
container_start_page 180
container_title Microbes and Environments
container_volume 30
creator Tang, Xiangming
Xie, Guijuan
Shao, Keqiang
Dai, Jiangyu
Chen, Yuangao
Xu, Qiujin
Gao, Guang
description Oligosaline lakes in arid regions provide indispensable water resources for humans; however, information on the bacterial community composition (BCC) of this ecosystem is limited. In the present study, we explored seasonal and vertical variations in BCC in Lake Bosten, a unique oligosaline lake (1.2‰ salinity) in arid, northwestern China, using denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. We obtained 544 clones and 98 operational taxonomic units (OTUs) from six clone libraries. The top 10 OTUs represented 59.4% of the entire bacterial community. Betaproteobacteria (22.1%), Gammaproteobacteria (19.9%), Bacteroidetes (18.8%), and Firmicutes (11.4%) dominated in Lake Bosten. Although seasonal variations were recorded in BCC, the vertical changes observed were not significant. Water temperature and salinity were the most important factors shaping the dynamics of BCC. A low degree of overlap was observed in BCC between Lake Bosten and freshwater ecosystems, especially for Betaproteobacteria and Bacteroidetes. An RDP seqmatch analysis showed that 169 sequences (31%) were novel bacterial sequences (
doi_str_mv 10.1264/jsme2.ME14177
format article
fullrecord <record><control><sourceid>pubmedcentral_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4462929</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>pubmedcentral_primary_oai_pubmedcentral_nih_gov_4462929</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-4ada8e1b22ddb1a8073f915e7155b47a28fda77acc7fe0b1d9860f689e5c94503</originalsourceid><addsrcrecordid>eNpVkc-O0zAQxiMEYpeFI3e_QBb_jRMOSLTqwkpFvcDZmjiT1iWxI9u7VV-Bp6bbRpX2MjOa75vfHL6i-MzoPeOV_LJPI_L7XysmmdZvilsmpC6lpOrteeZlJRi7KT6ktKdUCKX5--KGq6ZWjaC3xb8F2IzRwUCWYRyfvMvHl2kKyWUXPHGebAa3DQkG55Gs4S-SRUgZ_VeyDgeyecY4wERCTxaYYYohY2hnKAHfkcuH4DrMmMjB5R15iJh2BzitycqGdDzhxvSxeNfDkPDT3O-KPw-r38uf5Xrz43H5fV1axUQuJXRQI2s577qWQU216BumUDOlWqmB130HWoO1ukfasq6pK9pXdYPKNlJRcVd8u3Cnp3bEzqLPEQYzRTdCPJoAzrxWvNuZbXg2Ula84c0JUF4ANoaUIvbXW0bNSyjmHIqZQzn5lxf_PmXY4tUNMTs74OwW1JzLfHVV7Q6iQS_-A62dnB4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Bacterial Community Composition in Oligosaline Lake Bosten: Low Overlap of Betaproteobacteria and Bacteroidetes with Freshwater Ecosystems</title><source>Open Access: PubMed Central</source><source>EZB Electronic Journals Library</source><creator>Tang, Xiangming ; Xie, Guijuan ; Shao, Keqiang ; Dai, Jiangyu ; Chen, Yuangao ; Xu, Qiujin ; Gao, Guang</creator><creatorcontrib>Tang, Xiangming ; Xie, Guijuan ; Shao, Keqiang ; Dai, Jiangyu ; Chen, Yuangao ; Xu, Qiujin ; Gao, Guang</creatorcontrib><description>Oligosaline lakes in arid regions provide indispensable water resources for humans; however, information on the bacterial community composition (BCC) of this ecosystem is limited. In the present study, we explored seasonal and vertical variations in BCC in Lake Bosten, a unique oligosaline lake (1.2‰ salinity) in arid, northwestern China, using denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. We obtained 544 clones and 98 operational taxonomic units (OTUs) from six clone libraries. The top 10 OTUs represented 59.4% of the entire bacterial community. Betaproteobacteria (22.1%), Gammaproteobacteria (19.9%), Bacteroidetes (18.8%), and Firmicutes (11.4%) dominated in Lake Bosten. Although seasonal variations were recorded in BCC, the vertical changes observed were not significant. Water temperature and salinity were the most important factors shaping the dynamics of BCC. A low degree of overlap was observed in BCC between Lake Bosten and freshwater ecosystems, especially for Betaproteobacteria and Bacteroidetes. An RDP seqmatch analysis showed that 169 sequences (31%) were novel bacterial sequences (&lt;97% similarity to the closest sequences in GenBank), which suggested that specific indigenous bacteria inhabit this oligosaline environment. Our results support bacterial endemicity being more common than previously considered, particularly in oligosaline lakes. An analysis of these communities may reveal how bacteria respond to increases in salinity and nutrients in the early stage of salinization and eutrophication.</description><identifier>ISSN: 1342-6311</identifier><identifier>EISSN: 1347-4405</identifier><identifier>DOI: 10.1264/jsme2.ME14177</identifier><identifier>PMID: 25985930</identifier><language>eng</language><publisher>Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</publisher><subject>bacterial community composition ; diversity ; Lake Bosten ; oligosaline ; salinity ; seasonal dynamics</subject><ispartof>Microbes and Environments, 2015, Vol.30(2), pp.180-188</ispartof><rights>2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions</rights><rights>Copyright 2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-4ada8e1b22ddb1a8073f915e7155b47a28fda77acc7fe0b1d9860f689e5c94503</citedby><cites>FETCH-LOGICAL-c513t-4ada8e1b22ddb1a8073f915e7155b47a28fda77acc7fe0b1d9860f689e5c94503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462929/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462929/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Tang, Xiangming</creatorcontrib><creatorcontrib>Xie, Guijuan</creatorcontrib><creatorcontrib>Shao, Keqiang</creatorcontrib><creatorcontrib>Dai, Jiangyu</creatorcontrib><creatorcontrib>Chen, Yuangao</creatorcontrib><creatorcontrib>Xu, Qiujin</creatorcontrib><creatorcontrib>Gao, Guang</creatorcontrib><title>Bacterial Community Composition in Oligosaline Lake Bosten: Low Overlap of Betaproteobacteria and Bacteroidetes with Freshwater Ecosystems</title><title>Microbes and Environments</title><addtitle>Microbes Environ.</addtitle><description>Oligosaline lakes in arid regions provide indispensable water resources for humans; however, information on the bacterial community composition (BCC) of this ecosystem is limited. In the present study, we explored seasonal and vertical variations in BCC in Lake Bosten, a unique oligosaline lake (1.2‰ salinity) in arid, northwestern China, using denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. We obtained 544 clones and 98 operational taxonomic units (OTUs) from six clone libraries. The top 10 OTUs represented 59.4% of the entire bacterial community. Betaproteobacteria (22.1%), Gammaproteobacteria (19.9%), Bacteroidetes (18.8%), and Firmicutes (11.4%) dominated in Lake Bosten. Although seasonal variations were recorded in BCC, the vertical changes observed were not significant. Water temperature and salinity were the most important factors shaping the dynamics of BCC. A low degree of overlap was observed in BCC between Lake Bosten and freshwater ecosystems, especially for Betaproteobacteria and Bacteroidetes. An RDP seqmatch analysis showed that 169 sequences (31%) were novel bacterial sequences (&lt;97% similarity to the closest sequences in GenBank), which suggested that specific indigenous bacteria inhabit this oligosaline environment. Our results support bacterial endemicity being more common than previously considered, particularly in oligosaline lakes. An analysis of these communities may reveal how bacteria respond to increases in salinity and nutrients in the early stage of salinization and eutrophication.</description><subject>bacterial community composition</subject><subject>diversity</subject><subject>Lake Bosten</subject><subject>oligosaline</subject><subject>salinity</subject><subject>seasonal dynamics</subject><issn>1342-6311</issn><issn>1347-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpVkc-O0zAQxiMEYpeFI3e_QBb_jRMOSLTqwkpFvcDZmjiT1iWxI9u7VV-Bp6bbRpX2MjOa75vfHL6i-MzoPeOV_LJPI_L7XysmmdZvilsmpC6lpOrteeZlJRi7KT6ktKdUCKX5--KGq6ZWjaC3xb8F2IzRwUCWYRyfvMvHl2kKyWUXPHGebAa3DQkG55Gs4S-SRUgZ_VeyDgeyecY4wERCTxaYYYohY2hnKAHfkcuH4DrMmMjB5R15iJh2BzitycqGdDzhxvSxeNfDkPDT3O-KPw-r38uf5Xrz43H5fV1axUQuJXRQI2s577qWQU216BumUDOlWqmB130HWoO1ukfasq6pK9pXdYPKNlJRcVd8u3Cnp3bEzqLPEQYzRTdCPJoAzrxWvNuZbXg2Ula84c0JUF4ANoaUIvbXW0bNSyjmHIqZQzn5lxf_PmXY4tUNMTs74OwW1JzLfHVV7Q6iQS_-A62dnB4</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Tang, Xiangming</creator><creator>Xie, Guijuan</creator><creator>Shao, Keqiang</creator><creator>Dai, Jiangyu</creator><creator>Chen, Yuangao</creator><creator>Xu, Qiujin</creator><creator>Gao, Guang</creator><general>Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</general><general>the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>2015</creationdate><title>Bacterial Community Composition in Oligosaline Lake Bosten: Low Overlap of Betaproteobacteria and Bacteroidetes with Freshwater Ecosystems</title><author>Tang, Xiangming ; Xie, Guijuan ; Shao, Keqiang ; Dai, Jiangyu ; Chen, Yuangao ; Xu, Qiujin ; Gao, Guang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-4ada8e1b22ddb1a8073f915e7155b47a28fda77acc7fe0b1d9860f689e5c94503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>bacterial community composition</topic><topic>diversity</topic><topic>Lake Bosten</topic><topic>oligosaline</topic><topic>salinity</topic><topic>seasonal dynamics</topic><toplevel>online_resources</toplevel><creatorcontrib>Tang, Xiangming</creatorcontrib><creatorcontrib>Xie, Guijuan</creatorcontrib><creatorcontrib>Shao, Keqiang</creatorcontrib><creatorcontrib>Dai, Jiangyu</creatorcontrib><creatorcontrib>Chen, Yuangao</creatorcontrib><creatorcontrib>Xu, Qiujin</creatorcontrib><creatorcontrib>Gao, Guang</creatorcontrib><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbes and Environments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Xiangming</au><au>Xie, Guijuan</au><au>Shao, Keqiang</au><au>Dai, Jiangyu</au><au>Chen, Yuangao</au><au>Xu, Qiujin</au><au>Gao, Guang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial Community Composition in Oligosaline Lake Bosten: Low Overlap of Betaproteobacteria and Bacteroidetes with Freshwater Ecosystems</atitle><jtitle>Microbes and Environments</jtitle><addtitle>Microbes Environ.</addtitle><date>2015</date><risdate>2015</risdate><volume>30</volume><issue>2</issue><spage>180</spage><epage>188</epage><pages>180-188</pages><issn>1342-6311</issn><eissn>1347-4405</eissn><abstract>Oligosaline lakes in arid regions provide indispensable water resources for humans; however, information on the bacterial community composition (BCC) of this ecosystem is limited. In the present study, we explored seasonal and vertical variations in BCC in Lake Bosten, a unique oligosaline lake (1.2‰ salinity) in arid, northwestern China, using denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. We obtained 544 clones and 98 operational taxonomic units (OTUs) from six clone libraries. The top 10 OTUs represented 59.4% of the entire bacterial community. Betaproteobacteria (22.1%), Gammaproteobacteria (19.9%), Bacteroidetes (18.8%), and Firmicutes (11.4%) dominated in Lake Bosten. Although seasonal variations were recorded in BCC, the vertical changes observed were not significant. Water temperature and salinity were the most important factors shaping the dynamics of BCC. A low degree of overlap was observed in BCC between Lake Bosten and freshwater ecosystems, especially for Betaproteobacteria and Bacteroidetes. An RDP seqmatch analysis showed that 169 sequences (31%) were novel bacterial sequences (&lt;97% similarity to the closest sequences in GenBank), which suggested that specific indigenous bacteria inhabit this oligosaline environment. Our results support bacterial endemicity being more common than previously considered, particularly in oligosaline lakes. An analysis of these communities may reveal how bacteria respond to increases in salinity and nutrients in the early stage of salinization and eutrophication.</abstract><pub>Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</pub><pmid>25985930</pmid><doi>10.1264/jsme2.ME14177</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1342-6311
ispartof Microbes and Environments, 2015, Vol.30(2), pp.180-188
issn 1342-6311
1347-4405
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4462929
source Open Access: PubMed Central; EZB Electronic Journals Library
subjects bacterial community composition
diversity
Lake Bosten
oligosaline
salinity
seasonal dynamics
title Bacterial Community Composition in Oligosaline Lake Bosten: Low Overlap of Betaproteobacteria and Bacteroidetes with Freshwater Ecosystems
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T19%3A44%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmedcentral_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20Community%20Composition%20in%20Oligosaline%20Lake%20Bosten:%20Low%20Overlap%20of%20Betaproteobacteria%20and%20Bacteroidetes%20with%20Freshwater%20Ecosystems&rft.jtitle=Microbes%20and%20Environments&rft.au=Tang,%20Xiangming&rft.date=2015&rft.volume=30&rft.issue=2&rft.spage=180&rft.epage=188&rft.pages=180-188&rft.issn=1342-6311&rft.eissn=1347-4405&rft_id=info:doi/10.1264/jsme2.ME14177&rft_dat=%3Cpubmedcentral_cross%3Epubmedcentral_primary_oai_pubmedcentral_nih_gov_4462929%3C/pubmedcentral_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c513t-4ada8e1b22ddb1a8073f915e7155b47a28fda77acc7fe0b1d9860f689e5c94503%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/25985930&rfr_iscdi=true