Positive regulation of neocortical synapse formation by the Plexin-D1 receptor

Abstract Synapse formation is a critical process during neural development and is coordinated by multiple signals. Several lines of evidence implicate the Plexin-D1 receptor in synaptogenesis. Studies have shown that Plexin-D1 signaling is involved in synaptic specificity and synapse formation in sp...

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Bibliographic Details
Published in:Brain research 2015-08, Vol.1616, p.157-165
Main Authors: Wang, F, Eagleson, K.L, Levitt, P
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion Molecules, Neuronal - genetics
Cell Adhesion Molecules, Neuronal - metabolism
Disks Large Homolog 4 Protein
Embryo, Mammalian
Female
Gene Expression Regulation, Developmental - genetics
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Guanylate Kinases - metabolism
Humans
Image analysis
In vitro
Intracortical
Membrane Proteins - metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Confocal
Mouse
Neocortex - cytology
Neurology
Neurons - physiology
Pregnancy
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
Synapses - genetics
Synapses - physiology
Synapsins - metabolism
Synaptogenesis
Transfection
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Summary:Abstract Synapse formation is a critical process during neural development and is coordinated by multiple signals. Several lines of evidence implicate the Plexin-D1 receptor in synaptogenesis. Studies have shown that Plexin-D1 signaling is involved in synaptic specificity and synapse formation in spinal cord and striatum. Expression of Plexin-D1 and its principal neural ligand, Sema3E, by neocortical neurons is temporally and spatially regulated, reaching the highest level at the time of synaptogenesis in mice. In this study, we examined the function of Plexin-D1 in synapse formation by primary neocortical neurons in vitro. A novel, automated image analysis method was developed to quantitate synapse formation under baseline conditions and with manipulation of Plexin-D1 levels. shRNA and overexpression manipulations caused opposite changes, with reduction resulting in less synapse formation, an effect distinct from that reported in the striatum. The data indicate that Plexin-D1 operates in a cell context-specific fashion, mediating different synaptogenic outcomes depending upon neuron type.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2015.05.005