High Rab27A expression indicates favorable prognosis in CRC

• Published in: Diagnostic pathology 2015-06, Vol.10 (1), p.68-68, Article 68
• Main Authors: Shi, Chuanbing, Yang, Xiaojun, Ni, Yijiang, Hou, Ning, Xu, Li, Zhan, Feng, Zhu, Huijun, Xiong, Lin, Chen, Pingsheng
• Format: Article
• Language: English
• Subjects: Aged; Analysis; Biomarkers, Tumor - analysis; Biomarkers, Tumor - genetics; Cell Differentiation; Chi-Square Distribution; Colectomy; Colorectal cancer; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - genetics; Colorectal Neoplasms - mortality; Colorectal Neoplasms - pathology; Colorectal Neoplasms - surgery; Development and progression; Female; Genetic aspects; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Male; Metastasis; Middle Aged; Multivariate Analysis; Physiological aspects; Predictive Value of Tests; Prognosis; Proportional Hazards Models; rab GTP-Binding Proteins - analysis; rab GTP-Binding Proteins - genetics; rab27 GTP-Binding Proteins; Real-Time Polymerase Chain Reaction; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; Risk Factors; RNA; RNA, Messenger - genetics; Time Factors; Treatment Outcome; Up-Regulation
• ISSN: 1746-1596; 1746-1596
• DOI: 10.1186/s13000-015-0303-3

Rab27A is a peculiar member in Rab family and has been suggested to play essential roles in the development of human cancers. However, the association between Rab27A expression and clinicopathological characteristics of colorectal cancer (CRC) has not been elucidated yet. One-step quantitative real-time polymerase chain reaction (qPCR) test with 18 fresh-frozen CRC samples and immunohistochemistry (IHC) analysis in 112 CRC cases were executed to evaluate the relationship between Rab27A expression and the clinicopathological features of CRC. Cox regression and Kaplan-Meier survival analyses were performed to identify the prognostic factors for 112 CRC patients. The results specified that the expression levels of Rab27A mRNA and protein were significantly higher in CRC tissues than that in matched non-cancerous tissues, in both qPCR test (p = 0.029) and IHC analysis (p = 0.020). The IHC data indicated that the Rab27A protein expression in CRC was statistically correlated with lymph node metastasis (p = 0.022) and TNM stage (p = 0.026). Cox multi-factor analysis and Kaplan-Meier method suggested Rab27A protein expression (p = 0.012) and tumor differentiation (p = 0.004) were significantly associated with the overall survival of CRC patients. The data indicated the differentiate expression of Rab27A in CRC tissues and matched non-cancerous tissues. Rab27A may be used as a valuable prognostic biomarker for CRC patients.