bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1- JNK Pathways

• Published in: International journal of biological sciences 2015-01, Vol.11 (7), p.845-859
• Main Authors: Shi, Hongxue, Cheng, Yi, Ye, Jingjing, Cai, Pingtao, Zhang, Jinjing, Li, Rui, Yang, Ying, Wang, Zhouguang, Zhang, Hongyu, Lin, Cai, Lu, Xianghong, Jiang, Liping, Hu, Aiping, Zhu, Xinbo, Zeng, Qiqiang, Fu, Xiaobing, Li, Xiaokun, Xiao, Jian
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Blotting, Western; Cell Movement - drug effects; Cell Polarity - physiology; Cell Proliferation - physiology; Fibroblast Growth Factor 2 - pharmacology; Fibroblasts - drug effects; Fibroblasts - physiology; Glucose - metabolism; Humans; MAP Kinase Signaling System - physiology; Microscopy, Fluorescence; Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; rac1 GTP-Binding Protein - metabolism; Reactive Oxygen Species - metabolism; Research Paper; RNA, Small Interfering - genetics; Skin - cytology; Wound Healing - physiology
• ISSN: 1449-2288; 1449-2288
• DOI: 10.7150/ijbs.11921

Fibroblasts play a pivotal role in the process of cutaneous wound repair, whereas their migratory ability under diabetic conditions is markedly reduced. In this study, we investigated the effect of basic fibroblast growth factor (bFGF) on human dermal fibroblast migration in a high-glucose environment. bFGF significantly increased dermal fibroblast migration by increasing the percentage of fibroblasts with a high polarity index and reorganizing F-actin. A significant increase in intracellular reactive oxygen species (ROS) was observed in dermal fibroblasts under diabetic conditions following bFGF treatment. The blockage of bFGF-induced ROS production by either the ROS scavenger N-acetyl-L-cysteine (NAC) or the NADPH oxidase inhibitor diphenylene iodonium chloride (DPI) almost completely neutralized the increased migration rate of dermal fibroblasts promoted by bFGF. Akt, Rac1 and JNK were rapidly activated by bFGF in dermal fibroblasts, and bFGF-induced ROS production and promoted dermal fibroblast migration were significantly attenuated when suppressed respectively. In addition, bFGF-induced increase in ROS production was indispensable for the activation of focal adhesion kinase (FAK) and paxillin. Therefore, our data suggested that bFGF promotes the migration of human dermal fibroblasts under diabetic conditions through increased ROS production via the PI3K/Akt-Rac1-JNK pathways.