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Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation

Early acute kidney injury (AKI) in severely burned patients predicts a high mortality that is multi-factorial. Hydrogen has been reported to alleviate organ injury via selective quenching of reactive oxygen species. This study investigated the potential protective effects of hydrogen against severe...

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Published in:Journal of translational medicine 2015-06, Vol.13 (1), p.183-183, Article 183
Main Authors: Guo, Song-Xue, Fang, Quan, You, Chuan-Gang, Jin, Yun-Yun, Wang, Xin-Gang, Hu, Xin-Lei, Han, Chun-Mao
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description Early acute kidney injury (AKI) in severely burned patients predicts a high mortality that is multi-factorial. Hydrogen has been reported to alleviate organ injury via selective quenching of reactive oxygen species. This study investigated the potential protective effects of hydrogen against severe burn-induced early AKI in rats. Severe burn were induced via immersing the shaved back of rats into a 100°C bath for 15 s. Fifty-six Sprague-Dawley rats were randomly divided into Sham, Burn + saline, and Burn + hydrogen-rich saline (HS) groups, and renal function and the apoptotic index were measured. Kidney histopathology and immunofluorescence staining, quantitative real-time PCR, ELISA and western blotting were performed on the sera or renal tissues of burned rats to explore the underlying effects and mechanisms at varying time points post burn. Renal function and tubular apoptosis were improved by HS treatment. In addition, the oxidation-reduction potential and malondialdehyde levels were markedly reduced with HS treatment, whereas endogenous antioxidant enzyme activities were significantly increased. HS also decreased the myeloperoxidase levels and influenced the release of inflammatory mediators in the sera and renal tissues of the burned rats. The regulatory effects of HS included the inhibition of p38, JNK, ERK and NF-κB activation, and an increase in Akt phosphorylation. Hydrogen can attenuate severe burn-induced early AKI; the mechanisms of protection include the inhibition of oxidative stress induced apoptosis and inflammation, which may be mediated by regulation of the MAPKs, Akt and NF-κB signalling pathways.
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In addition, the oxidation-reduction potential and malondialdehyde levels were markedly reduced with HS treatment, whereas endogenous antioxidant enzyme activities were significantly increased. HS also decreased the myeloperoxidase levels and influenced the release of inflammatory mediators in the sera and renal tissues of the burned rats. The regulatory effects of HS included the inhibition of p38, JNK, ERK and NF-κB activation, and an increase in Akt phosphorylation. 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source Publicly Available Content (ProQuest); PubMed Central
subjects Acute Kidney Injury - blood
Acute Kidney Injury - complications
Acute Kidney Injury - drug therapy
Acute Kidney Injury - pathology
Acute-Phase Proteins
Analysis
Animals
Antioxidants
Apoptosis
Apoptosis - drug effects
Blotting, Western
Burns - blood
Burns - complications
Burns - drug therapy
Burns - pathology
Creatinine - blood
Enzyme-linked immunosorbent assay
Enzymes
Health aspects
Hydrogen
Hydrogen - pharmacology
Hydrogen - therapeutic use
Immunohistochemistry
Inflammation
Inflammation - complications
Inflammation - pathology
Inflammation Mediators - metabolism
Kidney Tubules - drug effects
Kidney Tubules - pathology
Lipocalins - blood
Male
Mediation
Medical research
Medicine, Experimental
Models, Biological
Oxidative stress
Oxidative Stress - drug effects
Peroxidase - metabolism
Prognosis
Proto-Oncogene Proteins - blood
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Signal Transduction - drug effects
Sodium Chloride - pharmacology
Sodium Chloride - therapeutic use
title Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation
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