Correlation between cell-free mRNA expressions and PLGF protein level in severe preeclampsia

• Published in: BMC research notes 2015-06, Vol.8 (1), p.208-208, Article 208
• Main Authors: Pramatirta, Akhmad Yogi, Mose, Johannes, Effendi, Jusuf S, Krisnadi, Sofie Rifayani, Anwar, Anita Deborah, Fauziah, Prima Nanda, Gurnadi, Jeffry Iman, Rihibiha, Dwi Davidson
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Analysis; Cell-Free System; Complications and side effects; Development and progression; Female; Gene Expression Regulation; Health aspects; Hostages; Humans; Messenger RNA; Mortality; Placenta Growth Factor; Pre-Eclampsia - genetics; Preeclampsia; Pregnancy; Pregnancy Proteins - genetics; Pregnancy Proteins - metabolism; Risk factors; RNA, Messenger - genetics; RNA, Messenger - metabolism; Young Adult
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-015-1186-9

Preeclampsia is a major cause of morbidity and mortality, both maternal and perinatal. The etiology and pathophysiology of preeclampsia remain unknown. Research shows the implantation of the placenta in preeclampsia occurs due to incomplete angiogenic imbalance as one of the preeclampsia pathogenesis. PlGF is angiogenic protein which is synthesized in placenta by mRNA PlGF. When damage occurs, mRNA will be released from cell and form cell-free mRNA. This study aims to analyze the differences between the PlGF mRNA expression in severe preeclampsia and normal pregnancy as well as to measure the relationship between cell-free mRNA and levels of PlGF with the incidence of severe preeclampsia. The method used in this study is an observational analytic study with cross-sectional design. Blood samples were obtained from patients with preeclampsia and normal pregnancies as the controlling factors in accordance with inclusion and exclusion criterias. Examination of the PlGF level was measured by ELISA method and mRNA PIGF expression was measured by RT-PCR. Physical and laboratory examinations of patients were recorded and collected as data. Calculations were done by statistical analysis. Mean of the cell-free mRNA PlGF expression level in severe preeclampsia is 2.2983 ng/mL within the scale of 1.96-2.83 ng/mL and deviation standard of 0.1897. Using Pearson Analysis Test, the result shows that there is a positive correlation between cell-free mRNA expression and PlGF protein level in severe preeclampsia, with r = 0.640 dan p