Enzymatic Saccharification of Lignocellulosic Residues by Cellulases Obtained from Solid State Fermentation Using Trichoderma viride

The aim of this study was to verify the viability of lignocellulosic substrates to obtain renewable energy source, through characterization of the cellulolytic complex, which was obtained by solid state fermentation using Trichoderma viride. Enzymatic activity of the cellulosic complex was measured...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-9
Main Authors: Costa, Jorge Alberto Vieira, Colla, Luciane Maria, Prigol, Elenizi, Tibolla, Heloisa, Sartori, Tanara, Bertolin, Telma Elita
Format: Article
Language:English
Subjects:
Cellulase
Cellulases - genetics
Cellulases - metabolism
Cellulose
Colleges & universities
Engineering
Enzymes
Eucalyptus
Fermentation
Food
Fungi
Hydrogen-Ion Concentration
Laboratories
Lignin
Lignin - chemistry
Lignin - metabolism
Lignocellulose
Microbiological research
Microorganisms
Renewable Energy
Temperature
Trichoderma - genetics
Trichoderma - metabolism
Trichoderma viride
Variables
Variance analysis
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Summary:The aim of this study was to verify the viability of lignocellulosic substrates to obtain renewable energy source, through characterization of the cellulolytic complex, which was obtained by solid state fermentation using Trichoderma viride. Enzymatic activity of the cellulosic complex was measured during saccharification of substrates filter paper, eucalyptus sawdust, and corncob, and compared with the activity of commercial cellulase. The characterization of the enzymes was performed by a 22 Full Factorial Design, where the pH and temperature were the variables of study. Enzymatic saccharification of different substrates appearedviable until 12 to be viable until 12 h; after this period the activity decreased for both enzymatic forms (cellulolytic complex and commercial cellulase). The enzymatic activity of the commercial cellulase was favored with the use of corncob as substrate, while the cellulolytic complex does not show any difference in its specificity by the substrates studied. The largest activities of both enzymes were obtained in the temperature and pH range between 40°C and 50°C and 4.8 and 5.2, respectively. The cellulolytic complex obtained appeared to be viable for the saccharification of lignocellulosic residues compared with the commercial cellulase.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/342716