Epitope analysis of Ara h 2 and Ara h 6: characteristic patterns of IgE-binding fingerprints among individuals with similar clinical histories

Summary Background Ara h 2 and Ara h 6 are moderately homologous and highly potent peanut allergens. Objective To identify IgE‐binding linear epitopes of Ara h 6, compare them to those of Ara h 2, and to stratify binding based on clinical histories. Methods Thirty highly peanut‐allergic subjects wer...

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Bibliographic Details
Published in:Clinical and experimental allergy 2015-02, Vol.45 (2), p.471-484
Main Authors: Otsu, K., Guo, R., Dreskin, S. C.
Format: Article
Language:English
Subjects:
2S Albumins, Plant - chemistry
2S Albumins, Plant - immunology
Adolescent
Adult
Aged
allergens
Amino Acid Sequence
Animals
Antigens, Plant - chemistry
Antigens, Plant - immunology
Ara h 2
Ara h 6
Cell Line
Child
Cluster Analysis
Epitope Mapping
Epitopes - chemistry
Epitopes - immunology
Epitopes - metabolism
Glycoproteins - chemistry
Glycoproteins - immunology
Humans
IgE
Immunoglobulin E - immunology
Immunoglobulin E - metabolism
microarrays
Middle Aged
Models, Molecular
Molecular Sequence Data
peanut
Peanut Hypersensitivity - immunology
peptides
Peptides - chemistry
Peptides - immunology
Peptides - metabolism
Protein Array Analysis
Protein Binding - immunology
Protein Conformation
Rats
Young Adult
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Summary:Summary Background Ara h 2 and Ara h 6 are moderately homologous and highly potent peanut allergens. Objective To identify IgE‐binding linear epitopes of Ara h 6, compare them to those of Ara h 2, and to stratify binding based on clinical histories. Methods Thirty highly peanut‐allergic subjects were stratified by clinical history. Sera were diluted to contain the same amount of anti‐peanut IgE. IgE binding to overlapping 20‐mer peptides of Ara h 2 and Ara h 6 was assessed using microarrays. Results Each subject had a unique IgE‐binding fingerprint to peptides; these data were coalesced into epitope binding. IgE from subjects with a history of more severe reactions (n = 19) had a smaller frequency of binding events (BEs) for both Ara h 2 (52 BEs of 152 (19X8epitopes) possible BEs and Ara h 6 (13 BEs of 133 (19X7 epitopes) possible BEs) compared to IgE from those with milder histories (n = 11) (Ara h 2: 47 BEs of 88 (11X8 epitopes) possible BEs, P 
ISSN:0954-7894
1365-2222
DOI:10.1111/cea.12407