An automated imaging system for radiation biodosimetry

ABSTRACT We describe here an automated imaging system developed at the Center for High Throughput Minimally Invasive Radiation Biodosimetry. The imaging system is built around a fast, sensitive sCMOS camera and rapid switchable LED light source. It features complete automation of all the steps of th...

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Bibliographic Details
Published in:Microscopy research and technique 2015-07, Vol.78 (7), p.587-598
Main Authors: Garty, Guy, Bigelow, Alan W., Repin, Mikhail, Turner, Helen C., Bian, Dakai, Balajee, Adayabalam S., Lyulko, Oleksandra V., Taveras, Maria, Yao, Y. Lawrence, Brenner, David J.
Format: Article
Language:English
Subjects:
Assaying
Automated
Automation
Automation - methods
Blocking
Cytokinesis
dicentrics
Fish
fluorescence microscopy
Histones - metabolism
Humans
Image Processing, Computer-Assisted - instrumentation
Image Processing, Computer-Assisted - methods
Imaging
mBAND
micronuclei
Micronucleus Tests
Micronucleus, Germline - chemistry
Micronucleus, Germline - radiation effects
Microscopy
Platforms
Radiometry
sCMOS
γ-H2AX
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Summary:ABSTRACT We describe here an automated imaging system developed at the Center for High Throughput Minimally Invasive Radiation Biodosimetry. The imaging system is built around a fast, sensitive sCMOS camera and rapid switchable LED light source. It features complete automation of all the steps of the imaging process and contains built‐in feedback loops to ensure proper operation. The imaging system is intended as a back end to the RABiT—a robotic platform for radiation biodosimetry. It is intended to automate image acquisition and analysis for four biodosimetry assays for which we have developed automated protocols: The Cytokinesis Blocked Micronucleus assay, the γ‐H2AX assay, the Dicentric assay (using PNA or FISH probes) and the RABiT‐BAND assay. Microsc. Res. Tech. 78:587–598, 2015. © 2015 Wiley Periodicals, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.22512