Photoelectrocyclization Turn-On Probe for Organelle Specific Spatiotemporally Defined Live Cell Imaging

Photoactivatable fluorophores are useful tools in live cell imaging due to their potential for precise spatial and temporal control. In this report, we describe a new photoactivatable organelle specific live cell imaging probe based on a 6π-electrocyclization/oxidation mechanism. We show that this n...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2015-05, Vol.54 (22), p.6442-6446
Main Authors: Tran, Mai N., Chenoweth, David M.
Format: Article
Language:English
Online Access:Get full text
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Summary:Photoactivatable fluorophores are useful tools in live cell imaging due to their potential for precise spatial and temporal control. In this report, we describe a new photoactivatable organelle specific live cell imaging probe based on a 6π-electrocyclization/oxidation mechanism. We show that this new probe is water soluble, non-cytotoxic, cell permeable and useful for mitochondrial imaging. The probe displays large Stokes shifts in both pre-activated and activated forms, allowing simultaneous use with common dyes and fluorescent proteins. Sequential single cell activation experiments in dense cellular environments demonstrate high spatial precision and utility in single or multi cell labeling experiments.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201502403