Vascular endothelial growth factor antagonism restores epithelial barrier dysfunction via affecting zonula occludens proteins

Epithelial barrier dysfunction is important in the pathogenesis of asthma and allergic responses, and is therefore a therapeutic target. The aim of the present study was to investigate the effects of dexamethasone, a classic therapeutic agent, an anti-tumor necrosis factor agent (etanercept), which...

Full description

Saved in:
Bibliographic Details
Published in:Experimental and therapeutic medicine 2015-07, Vol.10 (1), p.362-368
Main Authors: YUKSEL, HASAN, YILMAZ, OZGE, KARAMAN, MERAL, FIRINCI, FATIH, TURKELI, AHMET, KANIK, ESRA TOPRAK, INAN, SEVINC
Format: Article
Language:English
Subjects:
Animals
Asthma
Cellular proteins
claudin
Dexamethasone
epithelial barrier
Hogs
Molecular targeted therapy
Monoclonal antibodies
mouse model
occludin
Patient outcomes
Permeability
Properties
Proteins
Studies
Tumor necrosis factor-TNF
Vascular endothelial growth factor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epithelial barrier dysfunction is important in the pathogenesis of asthma and allergic responses, and is therefore a therapeutic target. The aim of the present study was to investigate the effects of dexamethasone, a classic therapeutic agent, an anti-tumor necrosis factor agent (etanercept), which is used to treat difficult cases of asthma, and an anti-vascular endothelial growth factor (VEGF) agent (bevacizumab), which is an angiogenesis inhibitor, on zonula occludens (ZO) proteins in an experimental asthma model. The experimental model of asthma was developed using intraperitoneal (IP) and inhaled administration of ovalbumin in 38 BALB/c mice, which were divided into four groups. The control group (n=6) did not receive any treatment, while the four remaining groups (n=8 per group) received an IP injection of saline, etanercept, bevacizumab or dexamethasone, respectively. Occludin, claudin and junctional adhesion molecule (JAM) were immunohistochemically stained in the left middle lobe samples using an indirect avidin-peroxidase method, after which the staining was semiquantified with H-scores. Statistically significant differences were observed in the occludin, claudin and JAM H-scores among the four groups (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2015.2502