MicroED data collection and processing

MicroED, a method at the intersection of X‐ray crystallography and electron cryo‐microscopy, has rapidly progressed by exploiting advances in both fields and has already been successfully employed to determine the atomic structures of several proteins from sub‐micron‐sized, three‐dimensional crystal...

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Bibliographic Details
Published in:Acta crystallographica. Section A, Foundations and advances Foundations and advances, 2015-07, Vol.71 (4), p.353-360
Main Authors: Hattne, Johan, Reyes, Francis E., Nannenga, Brent L., Shi, Dan, de la Cruz, M. Jason, Leslie, Andrew G. W., Gonen, Tamir
Format: Article
Language:English
Subjects:
CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY
Constraining
Cryoelectron Microscopy - methods
cryo‐EM
Crystal structure
CRYSTALLOGRAPHY
Crystallography, X-Ray - methods
CRYSTALS
Data acquisition
Data collection
Data Collection - methods
ELECTRON DIFFRACTION
ELECTRONS
Foundations
MicroED
MICROSCOPY
nanocrystals
NANOSTRUCTURES
POTENTIALS
PROCESSING
PROTEINS
Proteins - chemistry
REDUCTION
RESOLUTION
SAMPLE PREPARATION
Three dimensional
Three dimensional models
X-rays
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Summary:MicroED, a method at the intersection of X‐ray crystallography and electron cryo‐microscopy, has rapidly progressed by exploiting advances in both fields and has already been successfully employed to determine the atomic structures of several proteins from sub‐micron‐sized, three‐dimensional crystals. A major limiting factor in X‐ray crystallography is the requirement for large and well ordered crystals. By permitting electron diffraction patterns to be collected from much smaller crystals, or even single well ordered domains of large crystals composed of several small mosaic blocks, MicroED has the potential to overcome the limiting size requirement and enable structural studies on difficult‐to‐crystallize samples. This communication details the steps for sample preparation, data collection and reduction necessary to obtain refined, high‐resolution, three‐dimensional models by MicroED, and presents some of its unique challenges.
ISSN:2053-2733
0108-7673
2053-2733
DOI:10.1107/S2053273315010669