Impedance characterization, degradation, and in vitro biocompatibility for platinum electrodes on BioMEMS

• Published in: Biomedical microdevices 2015-02, Vol.17 (1), p.24-11, Article 24
• Main Authors: Geninatti, Thomas, Bruno, Giacomo, Barile, Bernardo, Hood, R. Lyle, Farina, Marco, Schmulen, Jeffrey, Canavese, Giancarlo, Grattoni, Alessandro
• Format: Article
• Language: English
• Subjects: Atomic force microscopy; Biocompatibility; Biological and Medical Physics; Biomedical Engineering and Bioengineering; Biophysics; Cell Line; Degradation; Deposition; Electric Impedance; Electric potential; Electrodes; Electrodes, Implanted; Engineering; Engineering Fluid Dynamics; Fibroblasts - metabolism; Humans; Materials Testing; Nanostructure; Nanotechnology; Platinum; Platinum - chemistry; Silicon; Silicon - chemistry
• ISSN: 1387-2176; 1572-8781
• DOI: 10.1007/s10544-014-9909-6

Fine control of molecular transport through microfluidic systems can be obtained by modulation of an applied electrical field across channels with the use of electrodes. In BioMEMS designed for biological fluids and in vivo applications, electrodes must be biocompatible, biorobust and stable. In this work, the analysis and characterization of platinum (Pt) electrodes integrated on silicon substrates for biomedical applications are presented. Electrodes were incorporated on the surface of silicon chips by adhesion of laminated Pt foils or deposited at 30°, 45° or 90° angle by e-beam or physical vapor (sputtering) methods. Electrical and physical properties of the electrodes were quantified and evaluated using electrical impedance spectroscopy and modelling of the electrode-electrolyte interfaces. Electrode degradation in saline solution at pH 7.4 was tested at room temperature and under accelerated conditions (90 °C), both in the presence and absence of an applied electrical potential. Degradation was quantified using atomic force microscopy (AFM) and inductively coupled plasma mass spectroscopy (ICP-MS). Biocompatibility was assessed by MTT proliferation assay with human dermal fibroblasts. Results demonstrated that the deposited electrodes were biocompatible with negligible material degradation and exhibited electrochemical behavior similar to Pt foils, especially for e-beam deposited electrodes. Finally, Pt electrodes e-beam deposited on silicon nanofabricated nanochannel membranes were evaluated for controlled drug delivery applications. By tuning a low applied electrical potential (