Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function

Pericentriolar material (PCM) mediates the microtubule (MT) nucleation and anchoring activity of centrosomes. A scaffold organized by Centrosomin (Cnn) serves to ensure proper PCM architecture and functional changes in centrosome activity with each cell cycle. Here, we investigate the mechanisms tha...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 2015-07, Vol.210 (1), p.79-97
Main Authors: Lerit, Dorothy A, Jordan, Holly A, Poulton, John S, Fagerstrom, Carey J, Galletta, Brian J, Peifer, Mark, Rusan, Nasser M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Calmodulin-Binding Proteins
Cell cycle
Cell division
Cellular biology
Centrosome - metabolism
Chromosome Segregation
Drosophila melanogaster
Drosophila Proteins - metabolism
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - metabolism
Embryos
Genomic Instability
Homeodomain Proteins - metabolism
Insects
Interphase
Microtubules - metabolism
Mitosis
Molecular Sequence Data
Protein Interaction Domains and Motifs
Protein Transport
Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pericentriolar material (PCM) mediates the microtubule (MT) nucleation and anchoring activity of centrosomes. A scaffold organized by Centrosomin (Cnn) serves to ensure proper PCM architecture and functional changes in centrosome activity with each cell cycle. Here, we investigate the mechanisms that spatially restrict and temporally coordinate centrosome scaffold formation. Focusing on the mitotic-to-interphase transition in Drosophila melanogaster embryos, we show that the elaboration of the interphase Cnn scaffold defines a major structural rearrangement of the centrosome. We identify an unprecedented role for Pericentrin-like protein (PLP), which localizes to the tips of extended Cnn flares, to maintain robust interphase centrosome activity and promote the formation of interphase MT asters required for normal nuclear spacing, centrosome segregation, and compartmentalization of the syncytial embryo. Our data reveal that Cnn and PLP directly interact at two defined sites to coordinate the cell cycle-dependent rearrangement and scaffolding activity of the centrosome to permit normal centrosome organization, cell division, and embryonic viability.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.201503117