Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis

The functional relevance of synovial ectopic lymphoid neogenesis (ELN) in rheumatoid arthritis (RA) remains unknown. As ELN correlates with the degree of tissue inflammation, we investigated whether ELN was associated with specific cytokine profiles. Synovial ELN was determined by immunohistology an...

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Published in:Arthritis research & therapy 2015-07, Vol.17 (1), p.173-173, Article 173
Main Authors: Cañete, Juan D, Celis, Raquel, Yeremenko, Nataliya, Sanmartí, Raimon, van Duivenvoorde, Leonie, Ramírez, Julio, Blijdorp, Iris, García-Herrero, Carmen M, Pablos, José L, Baeten, Dominique L
Format: Article
Language:English
Subjects:
Aged
Analysis
Arthritis
Arthritis, Rheumatoid - pathology
B cells
Choristoma
Drug therapy
Enzyme-Linked Immunosorbent Assay
Enzymes
Female
Health aspects
Humans
Immunohistochemistry
Inflammation
Interleukin-17 - immunology
Interleukin-23 - immunology
Interleukins
Lymphoid Tissue
Male
Middle Aged
Real-Time Polymerase Chain Reaction
Rheumatoid factor
RNA
Synovial Membrane - pathology
Synovitis - immunology
Synovitis - pathology
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Summary:The functional relevance of synovial ectopic lymphoid neogenesis (ELN) in rheumatoid arthritis (RA) remains unknown. As ELN correlates with the degree of tissue inflammation, we investigated whether ELN was associated with specific cytokine profiles. Synovial ELN was determined by immunohistology and long CD21 isoform (CD21L) expression. Cytokine expression was determined by multiplex enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (PCR) as well as immunohistology in synovial fluid (SF) (n = 44) and tissue (ST) (n = 108), respectively. Production of ELN-associated chemokines by fibroblast-like synoviocytes (FLS) was studied in vitro. Screening analysis of SF by multiplex ELISA showed higher protein levels of interleukin (IL)-23 (p = 0.018) and IL-17F (p = 0.028) in ELN+ versus ELN- samples. Other cytokines, including IL-17A, IL-6, and tumor necrosis factor (TNF)-α, were not different. The association between IL-23 and ELN was not biased by disease activity or other clinical features and was confirmed by higher IL-23 mRNA expression in ELN+ versus ELN- ST samples (p = 0.030), a correlation between IL-23 and CD21L expression in the same samples (r = 0.70 p 
ISSN:1478-6354
1478-6362
1478-6354
DOI:10.1186/s13075-015-0688-0