Three recognition events at the branch‐site adenine

An adenosine at the branch site, the nucleophile for the first transesterification step of splicing, is nearly invariant in mammalian pre‐mRNA introns. The chemical groups on the adenine base were varied systematically and assayed for formation of early spliceosome complexes and execution of the fir...

Full description

Saved in:
Bibliographic Details
Published in:The EMBO journal 1996-03, Vol.15 (6), p.1392-1402
Main Authors: Query, C. C., Strobel, S. A., Sharp, P. A.
Format: Article
Language:English
Subjects:
Adenine
Animals
Base Sequence
Molecular Sequence Data
Nucleic Acid Conformation
Protein Binding
Ribonucleoprotein, U2 Small Nuclear - metabolism
RNA Precursors - metabolism
RNA Splicing
RNA, Messenger - metabolism
Spliceosomes - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An adenosine at the branch site, the nucleophile for the first transesterification step of splicing, is nearly invariant in mammalian pre‐mRNA introns. The chemical groups on the adenine base were varied systematically and assayed for formation of early spliceosome complexes and execution of the first and second steps of splicing. Recognition of constituents of the adenine is critical in formation of a U2 snRNP‐containing complex on a minimal branch‐site oligonucleotide. Furthermore, the efficiencies of the first and second chemical steps have different dependencies on the functional groups of the adenine. In total, the chemical groups on the adenine base at the branch site are differentially recognized during at least three different processes in the splicing of pre‐mRNA. Moreover, a protein, p14, interacts with the adenine in a base‐specific fashion and may mediate early recognition of this base.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1996.tb00481.x