Evaluation of KRAS Gene Expression and LCS6 Variant in Genomic and Cell-Free DNA of Iranian Women With Endometriosis

Since the activation of KRAS results in de novo endometriosis in mice, KRAS is regarded as a crucial gene in ectopic endometrial implantation. Recently, it has been reported that 31% of women with endometriosis have KRAS let-7 complementary binding site 6 single-nucleotide polymorphism (LCS6 SNP). T...

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Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2015-06, Vol.22 (6), p.679-684
Main Authors: Farahani, Maryam Shahrabi, Shahbazi, Shirin, Moghaddam, Soheila Amini, Mahdian, Reza
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Adult
Binding Sites
Case-Control Studies
Cell-Free System
Embryology
Endometriosis - diagnosis
Endometriosis - genetics
Endometriosis - metabolism
Female
Genetic Predisposition to Disease
Humans
Iran
Medicine & Public Health
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
Obstetrics/Perinatology/Midwifery
Original
Original Article
Phenotype
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins p21(ras)
ras Proteins - genetics
ras Proteins - metabolism
Real-Time Polymerase Chain Reaction
Reproductive Medicine
Reverse Transcriptase Polymerase Chain Reaction
Risk Factors
RNA, Messenger - genetics
RNA, Messenger - metabolism
Up-Regulation
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Summary:Since the activation of KRAS results in de novo endometriosis in mice, KRAS is regarded as a crucial gene in ectopic endometrial implantation. Recently, it has been reported that 31% of women with endometriosis have KRAS let-7 complementary binding site 6 single-nucleotide polymorphism (LCS6 SNP). This study addresses the correlation between KRAS LCS6 SNP and endometriosis in a case–control study. To detect probable somatic mutation in ectopic endometrial tissue, we evaluated LCS6 SNP in cell-free DNA samples. Quantitative real-time reverse transcription-polymerase chain reaction was performed to determine the expression of KRAS transcripts in eutopic endometrial tissue. Our results suggest that the variant is not associated with the development of endometriosis in Iranian women. We observed higher levels of KRAS messenger RNA (mRNA) expression in eutopic endometrium of patients with endometriosis compared to controls. Although, the KRAS LCS6 is neither constitutional nor somatic biomarker for endometriosis, increased expression ratio of KRAS mRNA indicates its role in the implantation of endometrial tissue outside the uterine cavity.
ISSN:1933-7191
1933-7205
DOI:10.1177/1933719114556478