Polymerase chain reaction: a sensitive method for detecting Helicobacter pylori infection in bleeding peptic ulcers

To assess the sensitivity and specificity of polymerase chain reaction (PCR) in detecting Helicobacter pylori (H pylori) infection in patients with bleeding peptic ulcers, and to compare its diagnostic efficacy with other invasive and non-invasive tests. From April to September 2002, H pylori status...

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Published in:World journal of gastroenterology : WJG 2005-07, Vol.11 (25), p.3909-3914
Main Authors: Lo, Ching-Chu, Lai, Kwok-Hung, Peng, Nan-Jing, Lo, Gin-Ho, Tseng, Hui-Hwa, Lin, Chiun-Ku, Shie, Chang-Bih, Wu, Chao-Ming, Chen, Yu-Shan, Huang, Wen-Keui, Chen, Angela, Hsu, Ping-I
Format: Article
Language:English
Subjects:
Aged
Brief Reports
Female
Helicobacter Infections - complications
Helicobacter Infections - diagnosis
Helicobacter pylori
Humans
Male
Middle Aged
Peptic Ulcer Hemorrhage - complications
Peptic Ulcer Hemorrhage - microbiology
Polymerase Chain Reaction - standards
Sensitivity and Specificity
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creator Lo, Ching-Chu
Lai, Kwok-Hung
Peng, Nan-Jing
Lo, Gin-Ho
Tseng, Hui-Hwa
Lin, Chiun-Ku
Shie, Chang-Bih
Wu, Chao-Ming
Chen, Yu-Shan
Huang, Wen-Keui
Chen, Angela
Hsu, Ping-I
description To assess the sensitivity and specificity of polymerase chain reaction (PCR) in detecting Helicobacter pylori (H pylori) infection in patients with bleeding peptic ulcers, and to compare its diagnostic efficacy with other invasive and non-invasive tests. From April to September 2002, H pylori status in 60 patients who consecutively presented with gastroduodenal ulcer bleeding was examined by rapid urease tests (RUT), histology, culture, PCR, serology and urea breath tests (UBT). The sensitivity of PCR was significantly higher than that of RUT, histology and culture (91% vs 66%, 43% and 37%, respectively; P = 0.01,
doi_str_mv 10.3748/wjg.v11.i25.3909
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From April to September 2002, H pylori status in 60 patients who consecutively presented with gastroduodenal ulcer bleeding was examined by rapid urease tests (RUT), histology, culture, PCR, serology and urea breath tests (UBT). The sensitivity of PCR was significantly higher than that of RUT, histology and culture (91% vs 66%, 43% and 37%, respectively; P = 0.01, <0.001, <0.001, respectively), but similar to that of serology (94%) and UBT (94%). Additionally, PCR exhibited a greater specificity than serology (100% vs 65%, P<0.01). However, the specificity of PCR did not differ from that of other tests. Further analysis revealed significant differences in the sensitivities of RUT, culture, histology and PCR between the patients with and those without blood in the stomach (P<0.01, P = 0.09, P<0.05, and P<0.05, respectively). PCR is the most accurate method among the biopsy-based tests to detect H pylori infection in patients with bleeding peptic ulcers. 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From April to September 2002, H pylori status in 60 patients who consecutively presented with gastroduodenal ulcer bleeding was examined by rapid urease tests (RUT), histology, culture, PCR, serology and urea breath tests (UBT). The sensitivity of PCR was significantly higher than that of RUT, histology and culture (91% vs 66%, 43% and 37%, respectively; P = 0.01, <0.001, <0.001, respectively), but similar to that of serology (94%) and UBT (94%). Additionally, PCR exhibited a greater specificity than serology (100% vs 65%, P<0.01). However, the specificity of PCR did not differ from that of other tests. Further analysis revealed significant differences in the sensitivities of RUT, culture, histology and PCR between the patients with and those without blood in the stomach (P<0.01, P = 0.09, P<0.05, and P<0.05, respectively). PCR is the most accurate method among the biopsy-based tests to detect H pylori infection in patients with bleeding peptic ulcers. Blood may reduce the sensitivities of all biopsy-based tests.]]></abstract><cop>United States</cop><pub>Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Veternans General Hospital, National Yang-Ming University, Taiwan, China%Department of Nuclear Medicine, Kaohsiung Veternans General Hospital, National Yang-Ming University, Taiwan, China%Department of Pathology, Kaohsiung Veternans General Hospital, National Yang-Ming University, Taiwan, China%Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Veternans General Hospital, National Yang-Ming University, Taiwan, China%Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, China</pub><pmid>15991292</pmid><doi>10.3748/wjg.v11.i25.3909</doi><tpages>6</tpages></addata></record>
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subjects Aged
Brief Reports
Female
Helicobacter Infections - complications
Helicobacter Infections - diagnosis
Helicobacter pylori
Humans
Male
Middle Aged
Peptic Ulcer Hemorrhage - complications
Peptic Ulcer Hemorrhage - microbiology
Polymerase Chain Reaction - standards
Sensitivity and Specificity
title Polymerase chain reaction: a sensitive method for detecting Helicobacter pylori infection in bleeding peptic ulcers
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