Ultrastructural and Morphalogical Changes of Mouse Ovarian Tissues Following Direct Cover Vitrification with Different Cryoprotectants

Cryopreservation of mammalian ovaries has been reported with different levels of success. Cryopreservation of ovarian tissue may be a potential alternative for treatment of infertility and many attempts have been done to improve the efficiency of ovarian cryopreservation. The objective of the presen...

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Published in:Journal of reproduction & infertility 2015-07, Vol.16 (3), p.138-147
Main Authors: Ghavami, Maryam, Mohammadnejad, Daryoush, Beheshti, Rahim, Solmani-Rad, Jafar, Abedelahi, Ali
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Reproductive system
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container_issue 3
container_start_page 138
container_title Journal of reproduction & infertility
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creator Ghavami, Maryam
Mohammadnejad, Daryoush
Beheshti, Rahim
Solmani-Rad, Jafar
Abedelahi, Ali
description Cryopreservation of mammalian ovaries has been reported with different levels of success. Cryopreservation of ovarian tissue may be a potential alternative for treatment of infertility and many attempts have been done to improve the efficiency of ovarian cryopreservation. The objective of the present study was to compare the direct cover vitrification (DCV) with ethylene glycol (EG), dimethyl sulfoxide (DMSO) and EG plus DMSO. Eighty five mice were sacrificed by cervical dislocation and their ovaries were cryopreserved in the presence of 5% EG or DMSO alone or as mixture, 10% EG or DMSO alone or as mixture and a group with ascending concentrations of cryoprotectants. After toxicity testing and vitrification warming, the ovaries were fixed for histological and ultrastructural studies. In addition, the viability of mechanically isolated follicles was studied by trypan blue staining. All data were compared by ANOVA (p
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Reproductive system
Rodents
Tissues
title Ultrastructural and Morphalogical Changes of Mouse Ovarian Tissues Following Direct Cover Vitrification with Different Cryoprotectants
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