The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats

Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional w...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-12
Main Authors: Elsayed, Fatma, Razek, Khalid A., Bary, Amany A., Attia, Noha, Nabil, Iman, Mehanna, Radwa A., Ahmed, Tamer A. E.
Format: Article
Language:English
Subjects:
Administration, Topical
Animals
Bone marrow
Bone Marrow Cells
Chronic Disease
Collagen
Culture Media, Conditioned - pharmacology
Dosage and administration
Extracellular matrix
Fibrin
Fibrin Tissue Adhesive - pharmacology
Growth factors
Male
Medical research
Medicine
Mesenchymal Stem Cells
Physiological aspects
Rats
Rats, Sprague-Dawley
Skin
Stem cells
Studies
Success
Wound Healing
Wounds and Injuries - therapy
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Summary:Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although—later—none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson’s trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM—via fibrin vehicle—could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/846062