Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples

We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichmen...

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Bibliographic Details
Published in:Journal of clinical microbiology 2015-08, Vol.53 (8), p.2731-2733
Main Authors: Lee, Tracy D, Adie, Kathleen, McNabb, Alan, Purych, Dale, Mannan, Kulvinder, Azana, Robert, Ng, Corrinne, Tang, Patrick, Hoang, Linda M N
Format: Article
Language:English
Subjects:
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Bacteriology
beta-Lactamases - analysis
beta-Lactamases - genetics
Epidemiological Monitoring
Gram-Negative Bacteria - enzymology
Gram-Negative Bacteria - genetics
Humans
Multiplex Polymerase Chain Reaction - methods
Real-Time Polymerase Chain Reaction - methods
Rectum - microbiology
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Description
Summary:We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01237-15