Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and...

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Bibliographic Details
Published in:Journal of clinical microbiology 2015-08, Vol.53 (8), p.2722-2726
Main Authors: Chan, Jasper Fuk-Woo, Choi, Garnet Kwan-Yue, Tsang, Alan Ka-Lun, Tee, Kah-Meng, Lam, Ho-Yin, Yip, Cyril Chik-Yan, To, Kelvin Kai-Wang, Cheng, Vincent Chi-Chung, Yeung, Man-Lung, Lau, Susanna Kar-Pui, Woo, Patrick Chiu-Yat, Chan, Kwok-Hung, Tang, Bone Siu-Fai, Yuen, Kwok-Yung
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Animals
Child
Child, Preschool
Coronavirus
Coronavirus - classification
Coronavirus - genetics
Coronavirus - isolation & purification
Coronavirus Infections - diagnosis
Coronavirus Infections - virology
Female
Humans
Infant
Male
Middle Aged
Oligonucleotide Probes - genetics
Oligonucleotides - genetics
Real-Time Polymerase Chain Reaction - methods
Reverse Transcriptase Polymerase Chain Reaction - methods
Sensitivity and Specificity
Virology
Young Adult
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Summary:Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01224-15