Platelet geometry sensing spatially regulates α-granule secretion to enable matrix self-deposition

Although the biology of platelet adhesion on subendothelial matrix after vascular injury is well characterized, how the matrix biophysical properties affect platelet physiology is unknown. Here we demonstrate that geometric orientation of the matrix itself regulates platelet α-granule secretion, a k...

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Bibliographic Details
Published in:Blood 2015-07, Vol.126 (4), p.531-538
Main Authors: Sakurai, Yumiko, Fitch-Tewfik, Jennifer L., Qiu, Yongzhi, Ahn, Byungwook, Myers, David R., Tran, Reginald, Fay, Meredith E., Ding, Lingmei, Spearman, Paul W., Michelson, Alan D., Flaumenhaft, Robert, Lam, Wilbur A.
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
Blood Platelets - cytology
Blood Platelets - metabolism
Case-Control Studies
Cell Membrane - metabolism
Cells, Cultured
Exocytosis - physiology
Fibrinogen - metabolism
Fibronectins - metabolism
Gray Platelet Syndrome - metabolism
Gray Platelet Syndrome - pathology
Humans
Immunoenzyme Techniques
Platelet Activation
Platelet Adhesiveness - physiology
Platelet Glycoprotein GPIIb-IIIa Complex - metabolism
Platelets and Thrombopoiesis
Pseudopodia
Wiskott-Aldrich Syndrome - metabolism
Wiskott-Aldrich Syndrome - pathology
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Summary:Although the biology of platelet adhesion on subendothelial matrix after vascular injury is well characterized, how the matrix biophysical properties affect platelet physiology is unknown. Here we demonstrate that geometric orientation of the matrix itself regulates platelet α-granule secretion, a key component of platelet activation. Using protein microcontact printing, we show that platelets spread beyond the geometric constraints of fibrinogen or collagen micropatterns with
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2014-11-607614