A new vaccine escape mutant of hepatitis B virus causes occult infection

There is growing public concern regarding assay sensitivity to HBsAg mutants in clinical diagnosis and vaccine escape. The aim of this study is to introduce a new HBsAg mutant strain. The serum samples were those of patient X at the age of 3 months and 3 years respectively, and of her mother immedia...

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Bibliographic Details
Published in:Human vaccines & immunotherapeutics 2015, Vol.11 (2), p.407-410
Main Authors: Ye, Qing, Shang, Shi-Qiang, Li, Wei
Format: Article
Language:English
Subjects:
Amino Acid Substitution
Animals
Child, Preschool
Computational Biology
DNA, Viral - genetics
Female
Hepatitis B - immunology
Hepatitis B - pathology
Hepatitis B - virology
Hepatitis B Surface Antigens - genetics
Hepatitis B Surface Antigens - immunology
Hepatitis B Vaccines - immunology
Hepatitis B virus - genetics
Hepatitis B virus - immunology
HEPATITIS/Case Report
Humans
Immune Evasion
Infant
Infant, Newborn
Sequence Analysis, DNA
Sequence Homology, Amino Acid
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Summary:There is growing public concern regarding assay sensitivity to HBsAg mutants in clinical diagnosis and vaccine escape. The aim of this study is to introduce a new HBsAg mutant strain. The serum samples were those of patient X at the age of 3 months and 3 years respectively, and of her mother immediately before parturition, which were used to amplify the HBsAg-coding DNA fragments by PCR. The HBsAg DNA sequences were translated into their corresponding amino acid sequences and then aligned in pubmed with nucleotide blast. The sequencing data of S coding regions shows that patient X has been infected by a new HBV variant with an A to C substitution at nt431, resulting in an Asp(GAC)to Ala(GCC) substitution at aa144 of major protein; CC to AA substitution at nt359 and nt360, resulting in an Pro(CCC) to Gln(CAA) substitution at aa120 of pre "a" epitope; A to G substitution at nt491, resulting in an Glu(GAG) to Gly(GGG) substitution at aa164 of post "a" epitope. Three new mutations (S171F, S174N and Q181R) at the antigenic epitopes of HBV presented by HLA class I molecules are found. The HBV mutant strain causes vaccine escape and occult infection.
ISSN:2164-5515
2164-554X
DOI:10.4161/21645515.2014.994461