Histone H4 acetylation required for chromatin decompaction during DNA replication

Faithful DNA replication is a prerequisite for cell proliferation. Several cytological studies have shown that chromosome structures alter in the S-phase of the cell cycle. However, the molecular mechanisms behind the alteration of chromosome structures associated with DNA replication have not been...

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Bibliographic Details
Published in:Scientific reports 2015-07, Vol.5 (1), p.12720-12720, Article 12720
Main Authors: Ruan, Kun, Yamamoto, Takaharu G., Asakawa, Haruhiko, Chikashige, Yuji, Kimura, Hiroshi, Masukata, Hisao, Haraguchi, Tokuko, Hiraoka, Yasushi
Format: Article
Language:English
Subjects:
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Acetylation
Arginine
Cell cycle
Cell proliferation
Chromatin
Chromatin Assembly and Disassembly - physiology
Compaction
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA Replication - physiology
DNA, Fungal - biosynthesis
DNA, Fungal - genetics
Eukaryotes
Histone acetyltransferase
Histone H4
Histones - genetics
Histones - metabolism
Humanities and Social Sciences
Lysine
Meiosis
Molecular modelling
multidisciplinary
Replication
S Phase - physiology
Schizosaccharomyces - genetics
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe Proteins - genetics
Schizosaccharomyces pombe Proteins - metabolism
Science
Yeast
Yeasts
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Summary:Faithful DNA replication is a prerequisite for cell proliferation. Several cytological studies have shown that chromosome structures alter in the S-phase of the cell cycle. However, the molecular mechanisms behind the alteration of chromosome structures associated with DNA replication have not been elucidated. Here, we investigated chromatin structures and acetylation of specific histone residues during DNA replication using the meiotic nucleus of the fission yeast Schizosaccharomyces pombe . The S. pombe meiotic nucleus provides a unique opportunity for measuring the levels of compaction of chromatin along the chromosome in a defined orientation. By direct measurement of chromatin compaction in living cells, we demonstrated that decompaction of chromatin occurs during meiotic DNA replication. This chromatin decompaction was suppressed by depletion of histone acetyltransferase Mst1 or by arginine substitution of specific lysine residues (K8 and K12) of histone H4. These results suggest that acetylation of histone H4 residues K8 and K12 plays a critical role in loosening chromatin structures during DNA replication.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep12720