Characterization of genetic diversity in chickpea using SSR markers, Start Codon Targeted Polymorphism (SCoT) and Conserved DNA-Derived Polymorphism (CDDP)

To evaluate the genetic diversity among 48 genotypes of chickpea comprising cultivars, landraces and internationally developed improved lines genetic distances were evaluated using three different molecular marker techniques: Simple Sequence Repeat (SSR); Start Codon Targeted (SCoT) and Conserved DN...

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Bibliographic Details
Published in:Physiology and molecular biology of plants 2015-07, Vol.21 (3), p.365-373
Main Authors: Hajibarat, Zahra, Saidi, Abbas, Hajibarat, Zohreh, Talebi, Reza
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Cell Biology
chickpeas
Cicer arietinum
cluster analysis
cultivars
DNA
genetic distance
genetic variation
genotype
germplasm conservation
landraces
Life Sciences
microsatellite repeats
Plant Physiology
Plant Sciences
Research Article
start codon
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Summary:To evaluate the genetic diversity among 48 genotypes of chickpea comprising cultivars, landraces and internationally developed improved lines genetic distances were evaluated using three different molecular marker techniques: Simple Sequence Repeat (SSR); Start Codon Targeted (SCoT) and Conserved DNA-derived Polymorphism (CDDP). Average polymorphism information content (PIC) for SSR, SCoT and CDDP markers was 0.47, 0.45 and 0.45, respectively, and this revealed that three different marker types were equal for the assessment of diversity amongst genotypes. Cluster analysis for SSR and SCoT divided the genotypes in to three distinct clusters and using CDDP markers data, genotypes grouped in to five clusters. There were positive significant correlation ( r  = 0.43, P 
ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-015-0306-2