Fabrication and characterization of injectable hydrogels derived from decellularized skeletal and cardiac muscle

•Injectable ECM hydrogels fabricated from decellularized porcine muscle.•Hydrogels are characterized for biochemical, structural, and mechanical properties.•ECM hydrogels can be used as minimally invasive scaffolds for tissue engineering. Biomaterials, which can contain appropriate biomechanical and...

Full description

Saved in:
Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2015-08, Vol.84, p.53-59
Main Authors: Ungerleider, J.L., Johnson, T.D., Rao, N., Christman, K.L.
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
Biomechanical Phenomena
Cell Separation
Decellularization
Extracellular matrix
Extracellular Matrix - chemistry
Glycosaminoglycans - chemistry
Hydrogel
Hydrogels - administration & dosage
Hydrogels - chemistry
Injections
Materials Testing
Muscle, Skeletal - chemistry
Muscle, Skeletal - cytology
Myocardial infarction
Myocardial Infarction - therapy
Myocardium - chemistry
Myocardium - cytology
Peripheral Arterial Disease - therapy
Peripheral artery disease
Sus scrofa
Tissue Engineering - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Injectable ECM hydrogels fabricated from decellularized porcine muscle.•Hydrogels are characterized for biochemical, structural, and mechanical properties.•ECM hydrogels can be used as minimally invasive scaffolds for tissue engineering. Biomaterials, which can contain appropriate biomechanical and/or biochemical cues, are increasingly being investigated as potential scaffolds for tissue regeneration and/or repair for treating myocardial infarction, heart failure, and peripheral artery disease. Specifically, injectable hydrogels are touted for their minimally invasive delivery, ability to self-assemble in situ, and capacity to encourage host tissue regeneration. Here we present detailed methods for fabricating and characterizing decellularized injectable cardiac and skeletal muscle extracellular matrix (ECM) hydrogels. The ECM derived hydrogels have low cellular and DNA content, retain sulfated glycosaminoglycans and other extracellular matrix proteins such as collagen, gel at physiologic temperature and pH, and assume a nanofibrous architecture. These injectable hydrogels are amenable to minimally invasive, tissue specific biomaterial therapies for treating myocardial infarction and peripheral artery disease.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2015.03.024