Antagonistic peptide technology for functional dissection of CLE peptides revisited

In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To o...

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Bibliographic Details
Published in:Journal of experimental botany 2015-08, Vol.66 (17), p.5367-5374
Main Authors: Czyzewicz, Nathan, Wildhagen, Mari, Cattaneo, Pietro, Stahl, Yvonne, Pinto, Karine Gustavo, Aalen, Reidunn B., Butenko, Melinka A., Simon, Rüdiger, Hardtke, Christian S., De Smet, Ive
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Gene Expression Regulation, Plant
Magnetic Resonance Spectroscopy - methods
Peptides - genetics
Peptides - metabolism
RESEARCH PAPER
Sequence Alignment
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Summary:In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a promising new tool, antagonistic peptide technology, was recently developed. Here, this antagonistic peptide technology was tested on selected CLE peptides and the related IDA peptide and its usefulness in the context of studies of peptide function discussed. Based on the analyses, it was concluded that the antagonistic peptide approach is not the ultimate means to overcome redundancy or lack of loss-of-function lines. However, information collected using antagonistic peptide approaches (in the broad sense) can be very useful, but these approaches do not work in all cases and require a deep insight on the interaction between the ligand and its receptor to be successful. This, as well as peptide ligand structure considerations, should be taken into account before ordering a wide range of synthetic peptide variants and/or generating transgenic plants.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erv284