The orphan receptor cDNA RDC7 encodes an A1 adenosine receptor

The extensive amino acid sequence conservation among G protein‐coupled receptors has been exploited to clone new members of this large family by homology screening or by PCR. Out of four such receptor cDNAs we cloned recently, RDC7 corresponds to a relatively abundant transcript in the brain cortex,...

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Bibliographic Details
Published in:The EMBO journal 1991-07, Vol.10 (7), p.1677-1682
Main Authors: Libert, F., Schiffmann, S.N., Lefort, A., Parmentier, M., Gérard, C., Dumont, J.E., Vanderhaeghen, J.J., Vassart, G.
Format: Article
Language:English
Subjects:
Adenosine - analogs & derivatives
Adenosine - pharmacology
Animals
Brain - drug effects
Brain - metabolism
Cell Line
Colforsin - pharmacology
Cricetinae
Cricetulus
Cyclic AMP - metabolism
DNA - isolation & purification
Dogs
genes
GTP-Binding Proteins - genetics
GTP-Binding Proteins - metabolism
Male
Receptors, Cell Surface - genetics
Receptors, Purinergic - genetics
Receptors, Purinergic P1
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Testis - drug effects
Testis - metabolism
thyroid
Thyroid Gland - drug effects
Thyroid Gland - metabolism
Tissue Distribution
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Summary:The extensive amino acid sequence conservation among G protein‐coupled receptors has been exploited to clone new members of this large family by homology screening or by PCR. Out of four such receptor cDNAs we cloned recently, RDC7 corresponds to a relatively abundant transcript in the brain cortex, the thyroid follicular cell and the testis. We have now identified RDC7 as an A1 adenosine receptor. The A1 agonist CPA [N6‐cyclopentyladenosine] decreased by 80% cAMP accumulation in forskolin‐stimulated CHO cells stably transfected with RDC7. Specific binding of another A1 adenosine agonist, [3H]CHA [N6‐cyclohexyladenosine], was demonstrated on membranes from Cos cells transfected with a pSVL construct harbouring the RDC7 cDNA insert. The binding characteristics were similar to those of the natural brain A1 receptor. The recombinant and the natural receptors behaved also in the same way in displacement experiments involving a series of A1 adenosine agonists. The binding characteristics of RDC7 were compared to those of RDC8, another orphan receptor recently identified as an A2 adenosine receptor. The two molecular species RDC7 and RDC8 correspond clearly to the A1 and A2 receptor entities defined hitherto on a purely pharmacological basis.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1991.tb07691.x