Structure of a novel InsP3 receptor

Inositol 1,4,5‐trisphosphate (InsP3) constitutes a major intracellular second messenger that transduces many growth factor and neurotransmitter signals. InsP3 causes the release of Ca2+ from intracellular stores by binding to specific receptors that are coupled to Ca2+ channels. One such receptor fr...

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Bibliographic Details
Published in:The EMBO journal 1991-11, Vol.10 (11), p.3199-3206
Main Authors: Südhof, T.C., Newton, C.L., Archer, B.T., Ushkaryov, Y.A., Mignery, G.A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
Blotting, Western
Calcium - metabolism
Calcium Channels
Cell receptors
Cell structures and functions
DNA - genetics
Fundamental and applied biological sciences. Psychology
Inositol 1,4,5-Trisphosphate Receptors
Miscellaneous
Molecular and cellular biology
Molecular Sequence Data
Rats
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Receptors, Cytoplasmic and Nuclear
RNA, Messenger - analysis
RNA, Messenger - genetics
Sequence Alignment
Substrate Specificity
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Summary:Inositol 1,4,5‐trisphosphate (InsP3) constitutes a major intracellular second messenger that transduces many growth factor and neurotransmitter signals. InsP3 causes the release of Ca2+ from intracellular stores by binding to specific receptors that are coupled to Ca2+ channels. One such receptor from cerebellum has previously been extensively characterized. We have now determined the full structure of a second, novel InsP3 receptor which we refer to as type 2 InsP3 receptor as opposed to the cerebellar type 1 InsP3 receptor. The type 2 InsP3 receptor has the same general structural design as the cerebellar type 1 InsP3 receptor with which it shares 69% sequence identity. Expression of the amino‐terminal 1078 amino acids of the type 2 receptor demonstrates high affinity binding of InsP3 to the type 2 receptor with a similar specificity but higher affinity than observed for the type 1 receptor. These results demonstrate the presence of several types of InsP3 receptor in brain and raise the possibility that intracellular Ca2+ signaling may involve multiple pathways with different regulatory properties dependent on different InsP3 receptors.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1991.tb04882.x