Axon‐regulated expression of a Schwann cell transcript that is homologous to a ‘growth arrest‐specific’ gene

We have isolated a 1.8 kb cDNA (pCD25) clone that encodes a transcript that is differentially expressed during nerve regeneration. Nucleotide sequence comparison indicates 89.6% homology with the recently identified murine ‘growth arrest‐specific’ gene gas3. The open reading frame of the CD25 transc...

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Bibliographic Details
Published in:The EMBO journal 1991-12, Vol.10 (12), p.3661-3668
Main Authors: Spreyer, P., Kuhn, G., Hanemann, C.O., Gillen, C., Schaal, H., Kuhn, R., Lemke, G., Müller, H.W.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Axons - metabolism
Base Sequence
Biological and medical sciences
Blotting, Northern
Cells, Cultured
DNA - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
genes
Immunohistochemistry
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
neurons
Nucleic Acid Hybridization
Rats
Rats, Inbred Strains
RNA, Messenger - genetics
Schwann Cells - metabolism
Sciatic Nerve - injuries
Sequence Homology, Nucleic Acid
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
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Summary:We have isolated a 1.8 kb cDNA (pCD25) clone that encodes a transcript that is differentially expressed during nerve regeneration. Nucleotide sequence comparison indicates 89.6% homology with the recently identified murine ‘growth arrest‐specific’ gene gas3. The open reading frame of the CD25 transcript predicts a 17 kDa protein with four putative transmembrane regions. Steady‐state levels of the CD25 mRNA are very much higher in sciatic nerve than in other tissues, and expression in sciatic nerve is confined to Schwann cells. Following nerve injury, the transcript levels rapidly declined in nerve segments distal to the site of lesion, but recovered upon nerve regeneration. In contrast, in distal stumps of permanently transected nerves, the mRNA level remained very low. Substantial amounts of the mRNA could be reinduced only upon anastomosis of these interrupted nerve stumps. Re‐induction of the mRNA followed the elongation of regenerating axons through the distal nerve segment. Our data indicate that axons regulate expression of the CD25 mRNA in Schwann cells, and suggest that the CD25 protein functions during Schwann cell growth and differentiation.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1991.tb04933.x