The use of next generation sequencing in the diagnosis and typing of respiratory infections

Highlights • We compared current viral respiratory diagnostic techniques with NGS. • NGS is able to detect respiratory viruses in clinical diagnostic samples. • With the current sample preparation method, NGS is less sensitive than RT-PCR. • NGS provided additional sequence and typing information co...

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Bibliographic Details
Published in:Journal of clinical virology 2015-08, Vol.69, p.96-100
Main Authors: Thorburn, Fiona, Bennett, Susan, Modha, Sejal, Murdoch, David, Gunson, Rory, Murcia, Pablo R
Format: Article
Language:English
Subjects:
Allergy and Immunology
Diagnostics
DNA, Viral - analysis
High-Throughput Nucleotide Sequencing - methods
Humans
Infectious Disease
Molecular Diagnostic Techniques - methods
Nasopharynx - virology
Next generation sequencing
Pilot Projects
Respiratory Tract Infections - diagnosis
Respiratory Tract Infections - virology
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Viral - analysis
Sensitivity and Specificity
Viral respiratory infection
Virus detection
Virus Diseases - diagnosis
Viruses - classification
Viruses - genetics
Viruses - isolation & purification
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Description
Summary:Highlights • We compared current viral respiratory diagnostic techniques with NGS. • NGS is able to detect respiratory viruses in clinical diagnostic samples. • With the current sample preparation method, NGS is less sensitive than RT-PCR. • NGS provided additional sequence and typing information compared with RT-PCR.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2015.06.082