Analysis of p16 gene mutations and their expression using exhaled breath condensate in non-small-cell lung cancer

The aim of the present study was to investigate the mutational status of exons 1 and 2 of the p16 gene in the exhaled breath condensate (EBC) of patients with non-small-cell lung cancer (NSCLC) and determine the feasibility and clinical significance of applying EBC in the diagnosis of NSCLC. Polymer...

Full description

Saved in:
Bibliographic Details
Published in:Oncology letters 2015-09, Vol.10 (3), p.1477-1480
Main Authors: CHEN, JIN-LIANG, CHEN, JIAN-RONG, HUANG, FEN-FEN, TAO, GUO-HUA, ZHOU, FENG, TAO, YI-JIANG
Format: Article
Language:English
Subjects:
Collectors
Deoxyribonucleic acid
DNA
exhaled breath condensate
Gender
Genetic testing
Lung cancer
Lymphatic system
Medical prognosis
Metastasis
Molecular biology
Mutation
non-small-cell lung cancer
Oncology
p16 gene
Patients
polymerase chain reaction
Software
Statistical analysis
Surgery
Thoracic surgery
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the present study was to investigate the mutational status of exons 1 and 2 of the p16 gene in the exhaled breath condensate (EBC) of patients with non-small-cell lung cancer (NSCLC) and determine the feasibility and clinical significance of applying EBC in the diagnosis of NSCLC. Polymerase chain reaction and DNA sequencing were applied to detect exon 1 and 2 alterations of the p16 gene in EBC by comparing 58 samples from NSCLC patients and 30 from healthy controls. Of the 58 EBC samples from NSCLC patients, 54 were successfully tested and 8 cases of mutations were identified, of which 3 were in exon 1 and 5 in exon 2. The mutation rate was 14.81% (8/54). There were no p16 gene mutations in the 30 samples obtained from healthy controls. EBC p16 gene mutations exhibited no statistically significant differences according to gender, smoking history, pathological type, degree of differentiation and presence or absence of lymph node metastasis. The p16 gene mutation rate was proportional to the tumor stage (P
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2015.3426