Macroscopic modeling of mammalian cell growth and metabolism

We review major modeling strategies and methods to understand and simulate the macroscopic behavior of mammalian cells. These strategies comprise two important steps: the first step is to identify stoichiometric relationships for the cultured cells connecting the extracellular inputs and outputs. In...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2015-09, Vol.99 (17), p.7009-7024
Main Authors: Ben Yahia, Bassem, Malphettes, Laetitia, Heinzle, Elmar
Format: Article
Language:English
Subjects:
Analysis
Animals
Batch processes
Biomedical and Life Sciences
Bioreactors
Biotechnology
Biotechnology industry
Cell culture
Cell growth
Cell Line
Cell Proliferation
Cells
cultured cells
Differential equations
Energy Metabolism
equations
Gene expression
Health aspects
Influence
Life Sciences
Mammals
Mathematical functions
Mathematical models
Metabolism
Metabolites
Microbial Genetics and Genomics
Microbiology
Mini-Review
Models, Biological
Monoclonal antibodies
Process controls
Studies
Vaccines
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Description
Summary:We review major modeling strategies and methods to understand and simulate the macroscopic behavior of mammalian cells. These strategies comprise two important steps: the first step is to identify stoichiometric relationships for the cultured cells connecting the extracellular inputs and outputs. In a second step, macroscopic kinetic models are introduced. These relationships together with bioreactor and metabolite balances provide a complete description of a system in the form of a set of differential equations. These can be used for the simulation of cell culture performance and further for optimization of production.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-015-6743-6