High-throughput sorting of drops in microfluidic chips using electric capacitance

• Published in: Biomicrofluidics 2015-07, Vol.9 (4), p.044116-044116
• Main Authors: Pit, Arjen M, de Ruiter, Riëlle, Kumar, Anand, Wijnperlé, Daniel, Duits, Michèl H G, Mugele, Frieder
• Format: Article
• Language: English
• Subjects: Electrical resistivity; Electrodes; Microfluidics; Ohmic dissipation; Regular; Resistance heating; Two phase flow
• ISSN: 1932-1058; 1932-1058
• DOI: 10.1063/1.4928452

We analyze a recently introduced approach for the sorting of aqueous drops with biological content immersed in oil, using a microfluidic chip that combines the functionality of electrowetting with the high throughput of two-phase flow microfluidics. In this electrostatic sorter, three co-planar electrodes covered by a thin dielectric layer are placed directly below the fluidic channel. Switching the potential of the central electrode creates an electrical guide that leads the drop to the desired outlet. The generated force, which deflects the drop, can be tuned via the voltage. The working principle is based on a contrast in conductivity between the drop and the continuous phase, which ensures successful operation even for drops of highly conductive biological media like phosphate buffered saline. Moreover, since the electric field does not penetrate the drop, its content is protected from electrical currents and Joule heating. A simple capacitive model allows quantitative prediction of the electrostatic forces exerted on drops. The maximum achievable sorting rate is determined by a competition between electrostatic and hydrodynamic forces. Sorting speeds up to 1200 per second are demonstrated for conductive drops of 160 pl in low viscosity oil.