Morphological and Molecular Characterization of Exophiala polymorpha sp. nov. Isolated from Sporotrichoid Lymphocutaneous Lesions in a Patient with Myasthenia Gravis

Exophiala species are capable of causing cutaneous and subcutaneous infections in immunocompromised patients. An Exophiala isolate was cultured from a biopsy specimen of a lesion on the forearm of a patient with myasthenia gravis. The patient also had lesions on the palm and distal aspects of the ha...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical microbiology 2015-09, Vol.53 (9), p.2816-2822
Main Authors: Yong, Lee K, Wiederhold, Nathan P, Sutton, Deanna A, Sandoval-Denis, Marcelo, Lindner, Jonathan R, Fan, Hongxin, Sanders, Carmita, Guarro, Josep
Format: Article
Language:English
Subjects:
Aged
Antifungal Agents - therapeutic use
Biopsy
Cluster Analysis
Dermatomycoses - diagnosis
Dermatomycoses - drug therapy
Dermatomycoses - microbiology
Dermatomycoses - pathology
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
Exophiala - classification
Exophiala - cytology
Exophiala - genetics
Exophiala - isolation & purification
Female
Histocytochemistry
Humans
Itraconazole - therapeutic use
Microbiological Techniques
Microscopy
Molecular Sequence Data
Myasthenia Gravis - complications
Mycology
Peptide Elongation Factor 1 - genetics
Phaeohyphomycosis - diagnosis
Phaeohyphomycosis - drug therapy
Phaeohyphomycosis - microbiology
Phaeohyphomycosis - pathology
Phylogeny
Sequence Analysis, DNA
Treatment Outcome
Tubulin - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Exophiala species are capable of causing cutaneous and subcutaneous infections in immunocompromised patients. An Exophiala isolate was cultured from a biopsy specimen of a lesion on the forearm of a patient with myasthenia gravis. The patient also had lesions on the palm and distal aspects of the hand, which were successfully treated with a long-term course of itraconazole. A detailed morphological and molecular characterization of the isolate was undertaken. Phylogenetic analysis of the internal transcribed spacer region and portions of the β-tubulin and translation elongation factor 1-alpha genes indicated that the isolate was a novel species closely related to but genetically distinct from species within the Exophiala spinifera clade; the name Exophiala polymorpha sp. nov. is proposed. Morphologically, E. polymorpha most closely resembles E. xenobiotica but it differs in possessing phialides bearing prominent, wide collarettes, and it does not produce chlamydospores.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00622-15