An optimized enrichment technique for the isolation of Arthrobacter bacteriophage species from soil sample isolates

Bacteriophage isolation from environmental samples has been performed for decades using principles set forth by pioneers in microbiology. The isolation of phages infecting Arthrobacter hosts has been limited, perhaps due to the low success rate of many previous isolation techniques, resulting in an...

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Bibliographic Details
Published in:Journal of visualized experiments 2015-04 (98)
Main Authors: Cross, Trevor, Schoff, Courtney, Chudoff, Dylan, Graves, Libby, Broomell, Haley, Terry, Katrina, Farina, Jennifer, Correa, Alexandra, Shade, David, Dunbar, David
Format: Article
Language:English
Subjects:
Arthrobacter - virology
Bacteriophages - isolation & purification
Environmental Sciences
Soil - chemistry
Soil Microbiology
Virology - methods
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Summary:Bacteriophage isolation from environmental samples has been performed for decades using principles set forth by pioneers in microbiology. The isolation of phages infecting Arthrobacter hosts has been limited, perhaps due to the low success rate of many previous isolation techniques, resulting in an underrepresented group of Arthrobacter phages available for study. The enrichment technique described here, unlike many others, uses a filtered extract free of contaminating bacteria as the base for indicator bacteria growth, Arthrobacter sp. KY3901, specifically. By first removing soil bacteria the target phages are not hindered by competition with native soil bacteria present in initial soil samples. This enrichment method has resulted in dozens of unique phages from several different soil types and even produced different types of phages from the same enriched soil sample isolate. The use of this procedure can be expanded to most nutrient rich aerobic media for the isolation of phages in a vast diversity of interesting host bacteria.
ISSN:1940-087X
1940-087X
DOI:10.3791/52781