Isolation, expansion and characterisation of mesenchymal stem cells from human bone marrow, adipose tissue, umbilical cord blood and matrix: a comparative study

The multipotent and immunosuppressive capacities of mesenchymal stem cells (MSCs) attract several scientists worldwide towards translational research focusing on treatment of diseases including liver failure. Though MSC’s have been isolated from different sources, researchers do not concur on the be...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2015-10, Vol.67 (5), p.793-807
Main Authors: Secunda, R., Vennila, Rosy, Mohanashankar, A. M., Rajasundari, M., Jeswanth, S., Surendran, R.
Format: Article
Language:English
Subjects:
Adipose tissue
Antibodies
Biochemistry
Biomedicine
Biotechnology
Body fat
Bone marrow
CD105 antigen
CD14 antigen
CD29 antigen
CD34 antigen
CD44 antigen
CD45 antigen
CD73 antigen
Cell growth
Chemistry
Chemistry and Materials Science
Cord blood
Flow cytometry
Liver diseases
Mesenchymal stem cells
Original Research
Penicillin
Stem cells
Umbilical cord
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Summary:The multipotent and immunosuppressive capacities of mesenchymal stem cells (MSCs) attract several scientists worldwide towards translational research focusing on treatment of diseases including liver failure. Though MSC’s have been isolated from different sources, researchers do not concur on the best source for expansion and clinical translation. In this study, we have compared the isolation, proliferation and expansion of MSCs from umbilical cord blood (UCB), Wharton’s Jelly (WJ), bone marrow (BM) and adipose tissue (AT). MSCs were isolated by density gradient separation from UCB, BM and AT and by both enzymatic and explant method for WJ. The MSCs are characterized by their ability to adhere to plastic, expression of positive (CD105, CD73, CD90, CD29, CD44) and negative (CD45, CD14, CD34) markers by flow cytometry and also by their in vitro adipogenic, osteogenic and chondrogenic differentiation. This comprehensive study clearly shows that WJ is better than UCB both in terms of rapidity, yield and ease of procedure. AT and BM are autologous sources for MSC’s but the specimen collection involves cumbersome and painful procedures and an invasive approach. However being autologous, they are safe and probable candidates for therapeutic future applications.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-014-9718-z