Luteolin Inhibits Angiotensin II-Stimulated VSMC Proliferation and Migration through Downregulation of Akt Phosphorylation

Luteolin is a naturally occurring flavonoid found in many plants that possesses cardioprotective properties. The purpose of this study was to elucidate the effect of luteolin on vascular smooth muscle cells (VSMCs) proliferation and migration induced by Angiotensin II (Ang II) and to investigate the...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2015-01, Vol.2015 (2015), p.1-9
Main Authors: Chen, Dan, Liu, Yang, Cao, Lijuan, Lang, Yasong, Li, Dongye, Zhu, Hong, Xu, Tongda, Wu, Wanling
Format: Article
Language:English
Subjects:
Angiotensin
Cell cycle
Disease
Gene expression
Kinases
Laboratory animals
Medical research
Migration
Pathogenesis
Phosphorylation
Polyclonal antibodies
Smooth muscle
Studies
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Summary:Luteolin is a naturally occurring flavonoid found in many plants that possesses cardioprotective properties. The purpose of this study was to elucidate the effect of luteolin on vascular smooth muscle cells (VSMCs) proliferation and migration induced by Angiotensin II (Ang II) and to investigate the mechanism(s) of action of this compound. Rat VSMCs were cultured in vitro, and the proliferation and migration of these cells following Ang II stimulation were monitored. Different doses of luteolin were added to VSMC cultures, and the proliferation and migration rate were observed by MTT and Transwell chamber assays, respectively. In addition, the expressions of p-Akt (308), p-Akt (473), and proliferative cell nuclear antigen (PCNA) in VSMCs were monitored by Western blotting. This study demonstrated that luteolin has an inhibitory effect on Ang II-induced VSMC proliferation and migration. Further, the levels of p-Akt (308), p-Akt (473), and PCNA were reduced in VSMCs treated with both Ang II and luteolin compared to VSMCs treated with only Ang II. These findings strongly suggest that luteolin inhibits Ang II-stimulated proliferation and migration of VSMCs, which is partially due to downregulation of the Akt signaling pathway.
ISSN:1741-427X
1741-4288
DOI:10.1155/2015/931782