Effect of Cnidium Lactone on Serum Mutant P53 and BCL-2/BAX Expression in Human Prostate Cancer Cells PC-3 Tumor-Bearing BALB/C Nude Mouse Model

Cnidium lactone is a natural coumarin compound that can inhibit a variety of cancer cell proliferation and induce cancer cell apoptosis. This experiment investigated the effect of cnidium lactone on molecular marker expression in prostate cancer nude mice to study its effect in inducing apoptosis. W...

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Bibliographic Details
Published in:Medical science monitor 2015-08, Vol.21, p.2421-2427
Main Authors: Bi, Dongbin, Yang, Mingshan, Zhao, Xia, Huang, Shiming
Format: Article
Language:English
Subjects:
Animal Study
Animals
Antineoplastic Agents, Phytogenic - administration & dosage
Antineoplastic Agents, Phytogenic - pharmacology
Apoptosis - drug effects
Basigin - blood
bcl-2-Associated X Protein - blood
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Cell Line, Tumor
Cnidium - chemistry
Coumarins - administration & dosage
Coumarins - pharmacology
Cyclophosphamide - pharmacology
Humans
Lactones - administration & dosage
Lactones - pharmacology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Mutant Proteins - blood
Mutant Proteins - genetics
Prostatic Neoplasms - blood
Prostatic Neoplasms - drug therapy
Prostatic Neoplasms - genetics
Proto-Oncogene Proteins c-bcl-2 - blood
Racemases and Epimerases - blood
Tumor Suppressor Protein p53 - blood
Tumor Suppressor Protein p53 - genetics
Xenograft Model Antitumor Assays
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Summary:Cnidium lactone is a natural coumarin compound that can inhibit a variety of cancer cell proliferation and induce cancer cell apoptosis. This experiment investigated the effect of cnidium lactone on molecular marker expression in prostate cancer nude mice to study its effect in inducing apoptosis. We randomly and equally divided 30 male BALB/C nude mice inoculated with human prostate cancer cells PC-3 into a negative control group, a cyclophosphamide group (500 mg/Kg), and cnidium lactone groups at 3 doses (280 mg/Kg, 140 mg/Kg, and 70 mg/Kg). The mice were weighed at 2 weeks after administration. Tunnel assay was applied to test the nude mice tumor cell apoptosis. ELISA was performed to detect serum AMACR, CD147, mutant P53, BCL-2, AND BAX expression levels. Tumor tissue was separated and weighed. Mice weight did not change significantly in the groups receiving 3 different doses of cnidium lactone(p>0.05), while it decreased obviously in the cyclophosphamide group (p
ISSN:1643-3750
1234-1010
1643-3750
DOI:10.12659/MSM.893745